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Research article - Peer-reviewed, 2020

Enhanced stress tolerance in transformed Ajuga bracteosa Wall. ex Benth. regenerants by upregulated gene expression of metabolic pathways

Rubnawaz, Samina; Kayani, Waqas Khan; Mahmood, Rashid; Mirza, Bushra

Abstract

The rol oncogenes of Agrobacterium rhizogenes enhance the production of medicinally important compounds in plants and provide a first barrier against the overproduction of reactive oxygen species during biotic and abiotic stress. 'this study was designed to evaluate the expression of genes involved in biosynthetic pathways and their impact on metabolic contents and environmental stress tolerance in regenerated Ajuga bracteosa Wall. ex Benth. After successful transformation, real-time quantitative PCR confirmed the increased expression (1.94-6.59-fold) of HMGR, HDS, FDS, PAL, and TTG1 genes in transgenic lines. Furthermore, GC-MS coupled with principal component analysis revealed diverse concentrations of 97 metabolites in A. bracteosa. Transgenic lines showed greater survival under multiple stresses. This was revealed by significant chlorophyll content (8.13-21 mu moles/m(2)), higher quantum efficiency of PSII (F-v/F-m), and the performance index (PIabs) value. Similarly, catalase and peroxidase enzyme activities were enhanced during extreme drought (300-400 mM mannitol) and salinity (150-200 mM NaCl) conditions, compared to untransformed control. Wild type control plant leaves were completely necrotized by Aspergillus fumigatus (FCBP 66) and Fusarium solani (FCBP 0291), whereas transformed leaves had improved antifungal resistance. In conclusion, our data suggest that rolABC genes have a significant impact on the synthesis of metabolites involved in enhancing multistress tolerance in A. bracteosa.

Keywords

Ajuga bracteosa; rolABC; real-time quantitative PCR; GC-MS; principal component analysis; biotic and abiotic stress

Published in

Turkish Journal Of Botany
2020, volume: 44, number: 4, pages: 410-426
Publisher: TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY

Authors' information

Rubnawaz, Samina
Quaid I Azam Univ
Kayani, Waqas Khan
Swedish University of Agricultural Sciences, Department of Plant Breeding
Mahmood, Rashid
Quaid I Azam Univ
Mirza, Bushra
Quaid I Azam Univ

UKÄ Subject classification

Botany

Publication Identifiers

DOI: https://doi.org/10.3906/bot-2002-13

URI (permanent link to this page)

https://res.slu.se/id/publ/107153