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Abstract

Centrifugation of boar semen through one layer of 40% colloid (Porcicoll) was previously shown to separate spermatozoa from bacteria without having a detrimental effect on sperm quality. However, some spermatozoa were lost. The purpose of the present study was to determine whether 20% or 30% Porcicoll could be used to recover most of the spermatozoa without impacting on sperm quality. Insemination doses (n = 10) from a commercial boar station were sent to the laboratory at the Swedish University of Agricultural Sciences and processed by Single Layer Centrifugation with 20% and 30% Porcicoll approximately 7 hr after semen collection. The resulting sperm samples and controls were evaluated for sperm quality immediately and again after storage at 16-18 degrees C for 4 and 7 days. Sperm recovery was 94 +/- 18% and 87 +/- 15% for 20% and 30% Porcicoll, respectively (p > .05). Sperm mitochondrial membrane potential and chromatin integrity were unaffected (p > .05). The proportion of live spermatozoa producing superoxide (9 +/- 8%, 7 +/- 6% and 3 +/- 1%;p < .05), and the proportion of spermatozoa with high stainability DNA (0.68 +/- 19%, 0.61 +/- 0.22% and 0.96 +/- 0.23%;p < .05-

Keywords

antimicrobial usage; artificial insemination doses; colloid centrifugation; membrane integrity; sperm chromatin

Published in

Reproduction in Domestic Animals
2020, volume: 55, number: 10, pages: 1337-1342
Publisher: WILEY

SLU Authors

UKÄ Subject classification

Genetics and Breeding in Agricultural Sciences

Publication identifier

  • DOI: https://doi.org/10.1111/rda.13779

Permanent link to this page (URI)

https://res.slu.se/id/publ/107164