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Research article - Peer-reviewed, 2020

Seed characterization and early nitrogen metabolism performance of seedlings from Altiplano and coastal ecotypes of Quinoa

Pinto-Irish, Katherine; Coba de la Pena, Teodoro; Ostria-Gallardo, Enrique; Ibanez, Cristian; Briones, Vilbett; Vergara, Alexander; Alvarez, Rodrigo; Castro, Catalina; Sanhueza, Carolina; Castro, Patricio A.; Bascunan-Godoy, Luisa

Abstract

Background Early seed germination and a functional root system development during establishment are crucial attributes contributing to nutrient competence under marginal nutrient soil conditions.Chenopodium quinoaWilld (Chenopodiaceae) is a rustic crop, able to grow in marginal areas. Altiplano and Coastal/Lowlands are two representative zones of quinoa cultivation in South America with contrasting soil fertility and edaphoclimatic conditions. In the present work, we hypothesize that the ecotypes of Quinoa from Altiplano (landrace Socaire) and from Coastal/Lowland (landrace Faro) have developed differential adaptive responses in order to survive under conditions of low availability of N in their respective climatic zones of Altiplano and Lowlands. In order to understand intrinsic differences for N competence between landraces, seed metabolite profile and germinative capacity were studied. Additionally, in order to elucidate the mechanisms of N uptake and assimilation at limiting N conditions during establishment, germinated seeds of both landraces were grown at either sufficient nitrate (HN) or low nitrate (LN) supply. We studied the photosynthetic performance, protein storage, root morphometrical parameters, activity and expression of N-assimilating enzymes, and the expression of nitrate transporters of roots in plants submitted to the different treatments. Results Seeds from Socaire landrace presented higher content of free N-related metabolites and faster seed germination rate compared to Faro landrace. Seedlings of both ecotypes presented similar physiological performance at HN supply, but at LN supply their differences were exalted. At LN, Socaire plants showed an increased root biomass (including a higher number and total length of lateral roots), a differential regulation of a nitrate transporter (aNPF6.3-like homologue) belonging to the Low Affinity Transport System (LATS), and an upregulation of a nitrate transporter (aNRT2.1-like homologue) belonging to the High Affinity nitrate Transport System (HATS) compared to Faro. These responses as a whole could be linked to a higher amount of stored proteins in leaves, associated to an enhanced photochemical performance in Altiplano plants, in comparison to Lowland quinoa plants. Conclusions These differential characteristics of Socaire over Faro plants could involve an adaptation to enhanced nitrate uptake under the brutal unfavorable climate conditions of Altiplano.

Keywords

Nitrogen metabolism; N transport; Glutamine synthetase; Photochemical process; Chlorophyll fluorescence; Nitrate reductase; Nitrate transporters; Nitrate-sensing; Proteins; Crop yield

Published in

BMC Plant Biology
2020, volume: 20, number: 1, article number: 343
Publisher: BMC

Authors' information

Pinto-Irish, Katherine
Ctr Estudios Avanzados Zonas Aridas CEAZA
Coba de la Pena, Teodoro
Ctr Estudios Avanzados Zonas Aridas CEAZA
Ostria-Gallardo, Enrique
Ctr Estudios Avanzados Zonas Aridas CEAZA
Ibanez, Cristian
Univ La Serena
Briones, Vilbett
Univ La Serena
Vergara, Alexander
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Alvarez, Rodrigo
Univ Santo Tomas
Castro, Catalina
Univ Concepcion
Sanhueza, Carolina
Univ Concepcion
Castro, Patricio A.
Univ Concepcion
Bascunan-Godoy, Luisa
Ctr Estudios Avanzados Zonas Aridas CEAZA

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1186/s12870-020-02542-w

URI (permanent link to this page)

https://res.slu.se/id/publ/107188