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Research article - Peer-reviewed, 2020

Diazotroph Genomes and Their Seasonal Dynamics in a Stratified Humic Bog Lake

Fernandez, Leyden; Peura, Sari; Eiler, Alexander; Linz, Alexandra M.; McMahon, Katherine D.; Bertilsson, Stefan

Abstract

Aquatic N-fixation is generally associated with the growth and mass development ofCyanobacteriain nitrogen-deprived photic zones. However, sequenced genomes and environmental surveys suggest active aquatic N-fixation also by many non-cyanobacterial groups. Here, we revealed the seasonal variation and genomic diversity of potential N-fixers in a humic bog lake using metagenomic data andnifgene clusters analysis. Groups with diazotrophic operons were functionally divergent and includedCholorobi,Geobacter,Desulfobacterales,Methylococcales, andAcidobacteria. In addition tonifH(a gene that encodes the dinitrogenase reductase component of the molybdenum nitrogenase), we also identified sequences corresponding to vanadium and iron-only nitrogenase genes. Within theChlorobipopulation, the nitrogenase (nifH) cluster was included in a well-structured retrotransposon. Furthermore, the presence of light-harvesting photosynthesis genes implies that anoxygenic photosynthesis may fuel nitrogen fixation under the prevailing low-irradiance conditions. The presence ofrnfgenes (related to the expression of H+/Na+-translocating ferredoxin: NAD+ oxidoreductase) inMethylococcalesandDesulfobacteralessuggests that other energy-generating processes may drive the costly N-fixation in the absence of photosynthesis. The highly reducing environment of the anoxic bottom layer of Trout Bog Lake may thus also provide a suitable niche for active N-fixers and primary producers. While future studies on the activity of these potential N-fixers are needed to clarify their role in freshwater nitrogen cycling, the metagenomic data presented here enabled an initial characterization of previously overlooked diazotrophs in freshwater biomes.

Keywords

diazotrophs; N-fixation; lake; hypolimnion; nifHgene

Published in

Frontiers in Microbiology
2020, volume: 11, article number: 1500
Publisher: FRONTIERS MEDIA SA

Authors' information

Fernandez, Leyden
Uppsala Univ
Peura, Sari
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Eiler, Alexander
Univ Oslo
Linz, Alexandra M.
Univ Wisconsin
McMahon, Katherine D.
Univ Wisconsin
Uppsala University
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment

UKÄ Subject classification

Microbiology

Publication Identifiers

DOI: https://doi.org/10.3389/fmicb.2020.01500

URI (permanent link to this page)

https://res.slu.se/id/publ/107200