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Research article - Peer-reviewed, 2020

ETHYLENE RESPONSE FACTOR 115 integrates jasmonate and cytokinin signaling machineries to repress adventitious rooting in Arabidopsis

Lakehal, Abdellah; Dob, Asma; Rahneshan, Zahra; Novak, Ondrej; Escamez, Sacha; Alallaq, Sanaria; Strnad, Miroslav; Tuominen, Hannele; Bellini, Catherine

Abstract

Adventitious root initiation (ARI) is ade novoorganogenesis program and a key adaptive trait in plants. Several hormones regulate ARI but the underlying genetic architecture that integrates the hormonal crosstalk governing this process remains largely elusive. In this study, we use genetics, genome editing, transcriptomics, hormone profiling and cell biological approaches to demonstrate a crucial role played by the APETALA2/ETHYLENE RESPONSE FACTOR 115 transcription factor. We demonstrate that ERF115 functions as a repressor of ARI by activating the cytokinin (CK) signaling machinery. We also demonstrate thatERF115is transcriptionally activated by jasmonate (JA), an oxylipin-derived phytohormone, which represses ARI in NINJA-dependent and independent manners. Our data indicate that NINJA-dependent JA signaling in pericycle cells blocks early events of ARI. Altogether, our results reveal a previously unreported molecular network involving cooperative crosstalk between JA and CK machineries that represses ARI.

Keywords

adventitious rooting; AP2; ERF transcription factors; cytokinins; de novoorganogenesis; jasmonate

Published in

New Phytologist
2020, volume: 228, number: 5, pages: 1611-1626
Publisher: WILEY

Authors' information

Lakehal, Abdellah
Umea Univ
Dob, Asma
Umea Univ
Rahneshan, Zahra
Umea Univ
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
The Czech Academy of Sciences
Palacký University Olomouc
Escamez, Sacha
Umea Univ
Alallaq, Sanaria
Umea Univ
Strnad, Miroslav
Palacky Univ
Tuominen, Hannele
Umea Univ
Bellini, Catherine
Univ Paris Saclay

UKÄ Subject classification

Botany

Publication Identifiers

DOI: https://doi.org/10.1111/nph.16794

URI (permanent link to this page)

https://res.slu.se/id/publ/107255