Skip to main content
SLU publication database (SLUpub)

Research article2020Peer reviewedOpen access

Single nucleotide polymorphism analysis of the ITS2 region of two sympatric malaria mosquito species in Sweden:Anopheles daciae and Anopheles messeae

Lilja, T.; Eklof, D.; Jaenson, T. G. T.; Lindstrom, A.; Terenius, O.

Abstract

Four species of theAnopheles maculipenniscomplex have previously been recorded in Sweden. A recent addition to the complex isAnopheles daciae, which is considered to be closely related to, but distinct fromAnopheles messeae. The original designation ofAn. daciaewas based on five genetic differences (161, 165, 167, 362 and 382) in the second internal transcribed spacer (ITS) 2 of the ribosomal RNA. Further studies have shown that only two nucleotide differences (362 and 382) robustly separate the species. Thirty-threeAn. maculipenniscomplex mosquitoes were collected in the province of Uppland, Sweden. All wereAn. daciaebut showed double peaks for three variable positions (161, 165 and 167). When cloned, the intra-individual nucleotide variation was almost exclusively fixed with either TTC or AAT, originally diagnostic forAn. messaeandAn. daciae, respectively. To further investigate the intra-individual variation, nineAn. daciaeand 11An. messeaewere collected in southern Sweden and their ITS2 fragments were amplified and sequenced using Illumina MiSeq sequencing (Illumina, Inc., San Diego, CA, USA). For the diagnostic nucleotide 382 no intra-individual variation could be detected. However, although eachAn. daciaespecimen carried several ITS2 sequence variants for the four other nucleotides, there was no intra-individual variation in theAn. messeaespecimens.

Keywords

Anopheles maculipennis complex; species diagnosis; intra-individual variation; haplotypes; polymorphic

Published in

Medical and Veterinary Entomology
2020, Volume: 34, number: 3, pages: 364-368
Publisher: WILEY

      Sustainable Development Goals

      Ensure healthy lives and promote well-being for all at all ages

      UKÄ Subject classification

      Zoology
      Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

      Publication identifier

      DOI: https://doi.org/10.1111/mve.12436

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/107265