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Research article2001Peer reviewed

Ultraviolet and middle wavelength sensitive cone responses in the electroretinogram (ERG) of normal and Rpe65-/- mice

Ekesten, B; Gouras, P; Salchow, DJ

Abstract

Ultra-violet (UV) and middle wavelength sensitive (M) cone responses were identified in the ERG of normal and Rpe65 -/- mice using chromatic flashes and selective chromatic adaptation. In normal mice, the UV-cone response was as large as, or larger, in the presence of a bright yellow adapting light than it is in the presence of a dim white light. The M-cone response became undetectable in the presence of the yellow adapting light. Yellow adapting light initially reduced the UV response, but it recovered in 8-10 min. The M-cone response did not recover. UV-cone responses were undetectable in Rpe65 -/- mice. The M-cone response of young Rpe65 -/- mice was almost as large as in normal mice. A yellow adapting light only diminished this M-cone response. With age, the M-cone response further decreased in Rpe -/- mice. We show a pronounced loss of UV-cone function in Rpe65 -/- mice, which may be related to a defect UV-cones share with rods. The M-cone function is also affected already in young Rpe65 -/- mice. The transient effect of a yellow adapting light on the UV-cone response of normal mice is suggested to be neural, because it disappears during maintained light adaptation. (C) 2001 Elsevier Science Ltd. All rights reserved.

Keywords

mouse; Rpe65; electroretinogram; cones; ultra-violet

Published in

Vision Research
2001, Volume: 41, number: 19, pages: 2425-2433 Publisher: PERGAMON-ELSEVIER SCIENCE LTD

      SLU Authors

    • Ekesten, Björn

      • Department of Large Animal Clinical Sciences, Swedish University of Agricultural Sciences

    UKÄ Subject classification

    Ophthalmology

    Publication identifier

    DOI: https://doi.org/10.1016/S0042-6989(01)00140-7

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/107312