Löfgren, Maria
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Research article2021Peer reviewedOpen access
Lofgren, Maria; Larsson, Pia; Lindberg, Ronny; Hornaeus, Katarina; Tyden, Eva
Mild, moderate and severe equine asthma is a problem for equine welfare. The aetiology of the disease is not known in detail but is likely multi-factorial. One important factor may be inhaled dust which carries harmful substances which may be bioactivated and thus can lead to local inflammation in the airways. The aim of this study was to investigate gene expression and protein localisation of cytochrome P450 (CYP) enzymes, superoxide dismutase and glutathione-S-transferases (GST) involved in bioactivation and detoxification of harmful substances in lungs of horses with or without histological evidence of lower airway inflammation. Significantly lower gene expression of CYP2A13 and GSTM1 was observed in lungs from horses with histological evidence of lower airway inflammation compared with horses without. A higher expression, although not significant, was found for CYP1A1 in horses with histological evidence of lower airway inflammation. There were no differences in gene expression of GSTP1 and SOD3. The proteins were localised in the respiratory epithelium which is of relevance as a defence to local exposure of inhaled harmful substances. In conclusion, our study reports differential gene expression of enzymes involved in bioactivation and detoxification of foreign substances in the lungs of horses with histological evidence of lower airway inflammation compared with horses without.
CYP; equine asthma; gene expression; GST; immunohistochemistry; SOD
Veterinary Medicine and Science
2021, Volume: 7, number: 1, pages: 16-24 Publisher: WILEY
Clinical Science
DOI: https://doi.org/10.1002/vms3.331
https://res.slu.se/id/publ/107332