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Research article - Peer-reviewed, 2020

The environment and species affect gut bacteria composition in laboratory co-cultured Anopheles gambiae and Aedes albopictus mosquitoes

Saab, Sally A.; Dohna, Heinrich zu; Nilsson, Louise K. J.; Onorati, Piero; Nakhleh, Johnny; Terenius, Olle; Osta, Mike A.

Abstract

The midgut microbiota of disease vectors plays a critical role in the successful transmission of human pathogens. The environment influences the microbiota composition; however, the relative mosquito-species contribution has not been rigorously disentangled from the environmental contribution to the microbiota structure. Also, the extent to which the microbiota of the adult sugar food source and larval water can predict that of the adult midgut and vice versa is not fully understood. To address these relationships, larvae and adults of Anopheles gambiae and Aedes albopictus were either reared separately or in a co-rearing system, whereby aquatic and adult stages of both species shared the larval water and sugar food source, respectively. Despite being reared under identical conditions, clear intra- and interspecies differences in midgut microbiota-composition were observed across seven cohorts, collected at different time points over a period of eight months. Fitting a linear model separately for each OTU in the mosquito midgut showed that two OTUs significantly differed between the midguts of the two mosquito species. We also show an effect for the sugar food source and larval water on the adult midgut microbiota. Our findings suggest that the mosquito midgut microbiota is highly dynamic and controlled by multiple factors.

Published in

Scientific Reports
2020, volume: 10, number: 1, article number: 3352
Publisher: NATURE PUBLISHING GROUP

Authors' information

Saab, Sally A.
American University of Beirut
Dohna, Heinrich zu
American University of Beirut
Nilsson, Louise
Swedish University of Agricultural Sciences, Department of Ecology
Nilsson, Louise
Uppsala University
Swedish University of Agricultural Sciences, Department of Ecology
Nakhleh, Johnny
American University of Beirut
Swedish University of Agricultural Sciences, Department of Ecology
Uppsala University
Osta, Mike A.
American University of Beirut

UKÄ Subject classification

Ecology
Environmental Sciences

Publication Identifiers

DOI: https://doi.org/10.1038/s41598-020-60075-6

URI (permanent link to this page)

https://res.slu.se/id/publ/107738