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Research article - Peer-reviewed, 2020

Stream microbial communities and ecosystem functioning show complex responses to multiple stressors in wastewater

Burdon, Francis J.; Bai, Yaohui; Reyes, Marta; Tamminen, Manu; Staudacher, Philipp; Mangold, Simon; Singer, Heinz; Rasanen, Katja; Joss, Adriano; Tiegs, Scott D.; Jokela, Jukka; Eggen, Rik I. L.; Stamm, Christian;

Abstract

Multiple anthropogenic drivers are changing ecosystems globally, with a disproportionate and intensifying impact on freshwater habitats. A major impact of urbanization are inputs from wastewater treatment plants (WWTPs). Initially designed to reduce eutrophication and improve water quality, WWTPs increasingly release a multitude of micropollutants (MPs; i.e., synthetic chemicals) and microbes (including antibiotic-resistant bacteria) to receiving environments. This pollution may have pervasive impacts on biodiversity and ecosystem services. Viewed through multiple lenses of macroecological and ecotoxicological theory, we combined field, flume, and laboratory experiments to determine the effects of wastewater (WW) on microbial communities and organic-matter processing using a standardized decomposition assay. First, we conducted a mensurative experiment sampling 60 locations above and below WWTP discharges in 20 Swiss streams. Microbial respiration and decomposition rates were positively influenced by WW inputs via warming and nutrient enrichment, but with a notable exception: WW decreased the activation energy of decomposition, indicating a "slowing" of this fundamental ecosystem process in response to temperature. Second, next-generation sequencing indicated that microbial community structure below WWTPs was altered, with significant compositional turnover, reduced richness, and evidence of negative MP influences. Third, a series of flume experiments confirmed that although diluted WW generally has positive influences on microbial-mediated processes, the negative effects of MPs are "masked" by nutrient enrichment. Finally, transplant experiments suggested that WW-borne microbes enhance decomposition rates. Taken together, our results affirm the multiple stressor paradigm by showing that different aspects of WW (warming, nutrients, microbes, and MPs) jointly influence ecosystem functioning in complex ways. Increased respiration rates below WWTPs potentially generate ecosystem "disservices" via greater carbon evasion from streams and rivers. However, toxic MP effects may fundamentally alter ecological scaling relationships, indicating the need for a rapprochement between ecotoxicological and macroecological perspectives.

Keywords

biodiversity; carbon processing; cotton-strip assay; micropollutants; next-generation sequencing; nutrients; temperature; warming

Published in

Global Change Biology

2020, volume: 26, number: 11, pages: 6363-6382

Authors' information

Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Eawag, Swiss Federal Institute of Aquatic Science and Technology
Bai, Yaohui
Research Center for Eco-Environmental Sciences (RCEES)
Reyes, Marta
Swiss Federal Institute of Aquatic Science and Technology (EAWAG)
Tamminen, Manu
University of Turku
Staudacher, Philipp
Swiss Federal Institute of Aquatic Science and Technology (EAWAG)
Mangold, Simon
Swiss Federal Institute of Aquatic Science and Technology (EAWAG)
Singer, Heinz
Swiss Federal Institute of Aquatic Science and Technology (EAWAG)
Rasanen, Katja
ETH Zurich
Joss, Adriano
Swiss Federal Institute of Aquatic Science and Technology (EAWAG)
Jokela, Jukka
ETH Zurich
Eggen, Rik I. L.
ETH Zurich
Stamm, Christian
Swiss Federal Institute of Aquatic Science and Technology (EAWAG)

Sustainable Development Goals

SDG3 Good health and wellbeing
SDG6 Clean water
SDG11 Sustainable cities and communities
SDG15 Life on land

UKÄ Subject classification

Ecology
Environmental Sciences

Publication Identifiers

DOI: https://doi.org/10.1111/gcb.15302

URI (permanent link to this page)

https://res.slu.se/id/publ/107804