Research article - Peer-reviewed, 2020
Effects of season and single layer centrifugation on bull sperm quality in Thailand
Nongbua, Thanapol; Utta, Apirak; Am-in, Nutthee; Suwimonteerabutr, Junpen; Johannisson, Anders; Morrell, Jane M.Abstract
Objective: The aim of study was to investigate the effects of season and single layer centri-fugation (SLC) before cryopreservation on post-thaw bull sperm quality in Thailand.Methods: Semen was collected from 6 bulls (Bos indicus) in summer, rainy season and winter 2014 through 2016. Semen characteristics, sperm morphology, sperm kinematics, viability, chromatin structure and mitochondrial membrane were evaluated. Meteorological data were available from the local meteorological station;Results: Season had an effect on semen characteristics in the raw ejaculate, with higher proportions of normal spermatozoa and lower abnormalities in winter than in the other two seasons. Sperm kinematics, viability, DNA fragmentation index, and mitochondrial membrane potential were not different between seasons. Sperm samples selected by SLC had greater normal morphology and a lower proportion with bent tails than controls and higher values of progressive motility (PRO), beat cross frequency, linearity, straightness, wobble (WOB), and lower values of slow motility, velocity average path (VAP), velocity curved line, and amplitude of lateral head displacement than controls. In addition, SLC-selection had a favorable effect on PRO, VAP, and WOB that differed among seasons.Conclusion: Our results suggested that these bulls were well adapted to their location, with season having an effect on sperm morphology. Moreover, SLC could be used prior to cryo-preservation, regardless of season, to enhance normal morphology and kinematics of bull sperm samples without adversely affecting other parameters of sperm quality. However, there was considerable variation among bulls in DNA fragmentation index, mitochondrial membrane potential and sperm viability. In addition, SLC had a positive effect on sperm morphology and sperm kinematics, which could be expected to influence fertility.Keywords
Temperature; Humidity; Bovine Semen Quality; Colloid Centrifugation; Frozen SemenPublished in
Asian-Australasian journal of animal sciences2020, volume: 33, number: 9, pages: 1411-1420
Publisher: ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC
Authors' information
Nongbua, Thanapol
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Nongbua, Thanapol
Mahasarakham University
Am-in, Nutthee
Chulalongkorn University
Suwimonteerabutr, Junpen
Chulalongkorn University
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
UKÄ Subject classification
Clinical Science
Publication Identifiers
DOI: https://doi.org/10.5713/ajas.19.0624
URI (permanent link to this page)
https://res.slu.se/id/publ/107855