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Research article - Peer-reviewed, 2020

Cell-surface receptors enable perception of extracellular cytokinins

Antoniadi, Ioanna; Novak, Ondrej; Gelova, Zuzana; Johnson, Alexander; Plihal, Ondrej; Simersky, Radim; Mik, Vaclav; Vain, Thomas; Mateo-Bonmati, Eduardo; Karady, Michal; Pernisova, Marketa; Plackova, Lenka; Opassathian, Korawit; Hejatko, Jan; Robert, Stephanie; Friml, Jiri; Dolezal, Karel; Ljung, Karin; Turnbull, Colin

Abstract

Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses. The main site of cytokinin perception in plant cells is thought to be the endoplasmic reticulum where most cytokinin receptors localise. Here via the use of bioactive probes that cannot enter plant cells and super-resolution microscopy, Antoniadi et al. show that cytokinin can also be perceived at the plasma membrane.

Published in

Nature Communications
2020, volume: 11, number: 1, article number: 4284
Publisher: NATURE PUBLISHING GROUP

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Imperial College London
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Palacký University Olomouc
Gelova, Zuzana
Masaryk University Brno
Johnson, Alexander
Institute of Science and Technology - Austria
Plihal, Ondrej
Palacky University Olomouc
Simersky, Radim
Palacky University Olomouc
Mik, Vaclav
Palacky University Olomouc
Vain, Thomas
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Mateo-Bonmatí, Eduardo (Bonmati, Eduardo Mateo)
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Karady, Michal
Institute of Experimental Botany of the Czech Academy of Sciences
Pernisova, Marketa
Masaryk University Brno
Plackova, Lenka
Palacky University Olomouc
Opassathian, Korawit
Imperial College London
Hejatko, Jan
Masaryk University Brno
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Friml, Jiri
Institute of Science and Technology - Austria
Dolezal, Karel
Palacky University Olomouc
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Cell Biology

Publication Identifiers

DOI: https://doi.org/10.1038/s41467-020-17700-9

URI (permanent link to this page)

https://res.slu.se/id/publ/107881