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Research article - Peer-reviewed, 2020

New extraction method prior to screening of organic micropollutants in various biota matrices using liquid chromatography coupled to high-resolution time-of-flight mass spectrometry

Durig, Wiebke; Kintzi, Aaron; Golovko, Oksana; Wiberg, Karin; Ahrens, Lutz;

Abstract

A new extraction method with limited clean-up requirements prior to screening various matrices for organic micropollutants using liquid chromatography-high resolution mass spectrometry (LC-HRMS) for analysis was developed. First, the performance of three extraction methods (QuEChERS with SPE clean-up, ultrasonication with SPE clean-up, extraction without SPE clean-up) was tested, optimized, and compared using > 200 contaminants of emerging concern (CECs) together covering a wide range of physicochemical properties applicable for suspect and non-target screening in biota. White-tailed sea eagle (Haliaeetus albicilla) muscle tissue was used in method development and optimization. The method without SPE clean-up was then applied to European perch (Perca fluviatilis) muscle, heart, and liver tissues. The optimization and application of the method demonstrated a wide applicable domain of the novel extraction method regarding species, tissues, and chemicals. For future applications, the suitability of the method for suspect and non-target screening was tested. Overall, our extraction method appears to be sufficiently simple and broad (relatively non-discriminant) for use prior to analysis of CECs in various biota.

Keywords

Extraction method; Biota; Clean-up; LC-QToF-MS; Suspect and non-target screening; White-tailed sea eagle

Published in

Talanta

2020, volume: 219, article number: 121294
Publisher: ELSEVIER

Authors' information

Kintzi, Aaron
No organisation
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Dürig, Wiebke
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment

UKÄ Subject classification

Analytical Chemistry

Publication Identifiers

DOI: https://doi.org/10.1016/j.talanta.2020.121294

URI (permanent link to this page)

https://res.slu.se/id/publ/108034