New extraction method prior to screening of organic micropollutants in various biota matrices using liquid chromatography coupled to high-resolution time-of-flight mass spectrometry

Abstract

A new extraction method with limited clean-up requirements prior to screening various matrices for organic micropollutants using liquid chromatography-high resolution mass spectrometry (LC-HRMS) for analysis was developed. First, the performance of three extraction methods (QuEChERS with SPE clean-up, ultrasonication with SPE clean-up, extraction without SPE clean-up) was tested, optimized, and compared using > 200 contaminants of emerging concern (CECs) together covering a wide range of physicochemical properties applicable for suspect and non-target screening in biota. White-tailed sea eagle (Haliaeetus albicilla) muscle tissue was used in method development and optimization. The method without SPE clean-up was then applied to European perch (Perca fluviatilis) muscle, heart, and liver tissues. The optimization and application of the method demonstrated a wide applicable domain of the novel extraction method regarding species, tissues, and chemicals. For future applications, the suitability of the method for suspect and non-target screening was tested. Overall, our extraction method appears to be sufficiently simple and broad (relatively non-discriminant) for use prior to analysis of CECs in various biota.

Keywords

Extraction method; Biota; Clean-up; LC-QToF-MS; Suspect and non-target screening; White-tailed sea eagle

Published in

Talanta
2020, volume: 219, article number: 121294
Publisher: ELSEVIER

SLU Authors

• Golovko, Oksana

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    

• Wiberg, Karin

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    

• Ahrens, Lutz

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    

• Dürig, Wiebke

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Analytical Chemistry

Publication identifier

• DOI: https://doi.org/10.1016/j.talanta.2020.121294

Permanent link to this page (URI)

https://res.slu.se/id/publ/108034