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Research article2001Peer reviewed

Do the 16 mer, 5 '-GUGGUCUGAUGAGGCC-3 ' and the 25 mer, 5 '-GGCCGAAACUCGUAAGAGUCACCAC-3 ', form a hammerhead ribozyme structure in physiological conditions? An NMR and UV thermodynamic study

Zamaratski, Edouard; Trifonova, Anna ; Acharya, Parag; Isaksson, Johan; Maltseva, Tatiana; Chattopadhayaya, Jyoti

Abstract

In a wide range of salt concentrations, 10–30 mM phosphate buffer containing up to 0.5 M Li2SO4 and 300 mM NaCl, 7.5 mM Mg2+, pH 5.5–7.5, a mixture of the 16 mer and the 25 mer RNA strands does not form a hammerhead in any amount detectable by NMR at 600 MHz. The imino-, amino-, aromatic- and anomeric protons in the NMR spectra of both the 16 mer and the 25 mer RNA have been assigned separately. Both the 16 mer and the 25 mer RNA both take up very stable hairpin structures, and when mixed together there is no major change of conformation in neither oligo-RNA.

Published in

Nucleosides, Nucleotides and Nucleic Acids
2001, Volume: 20, number: 4-7, pages: 1219-1223

    UKÄ Subject classification

    Organic Chemistry

    Publication identifier

    DOI: https://doi.org/10.1081/NCN-100002522

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/108060