Research article - Peer-reviewed, 2021
Monitoring soil microbial communities using molecular tools:DNAextraction methods may offset long-term management effectsChangey, Frederique; Blaud, Aimeric; Pando, Anne; Herrmann, Anke M.; Lerch, Thomas Z.
AbstractDespite recent technological advances in molecular ecology, DNA extraction from soil remains a crucial step when quantifying and characterizing soil microbial communities. Potential biases could hamper fundamental understanding of the dynamic relationships between soil properties and microorganisms under different agricultural practices. In this study, we compared four different DNA extraction methods for their ability to discriminate microbial communities of an arable soil subjected to different fertilization managements for more than 50 years (Ultuna, Sweden). The abundance and the diversity of bacteria, archaea and fungi were studied using qPCR and molecular fingerprints, respectively. Overall, the choice of DNA extraction method had a more pronounced effect on the fungal and archaeal communities in comparison to bacterial ones. The assessment of the microbial diversity was more sensitive to DNA extraction methods in comparison with the quantification of the abundances. The DNA extraction method clearly affects the intensity of the correlations between the abundance and/or diversity of microbial communities and environmental variables (C, N and pH) according to the targeted taxon. This study highlights that long-term effects can be offset by biases in DNA extraction methods. HighlightsUsing different DNA extraction methods may alter soil microbial survey. Average DNA concentrations vary from 1 to 3 mu g.g(-1)according to the extraction method. Gene copies numbers vary up to 2 orders of magnitude among DNA extraction methods. Fungal communities diversity descriptors were the most affected by DNA extraction
Keywordsarchaea; bacteria; environmental DNA; fungi; long-term field experiment; soil organic matter
Published inEuropean Journal of Soil Science
2021, volume: 72, number: 2, pages: 1026-1041
Edinburgh Napier University
Swedish University of Agricultural Sciences, Department of Soil and Environment
Lerch, Thomas Z.
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