Abstract

Cross-linked hyaluronic acid (HA) hydrogels are used in many biomedical applications but their characterization in order to distinguish between physicochemical properties is challenging. Longitudinal (T-1) and transverse (T-2) relaxation times and diffusion coefficient (D) of water protons in diepoxide 1,4-butanediol diglycidyl ether (BDDE)-cross-linked HA hydrogels were analyzed by high-field NMR spectroscopy to distinguish between different physicochemical properties. Hydrogels of different degrees of modification and cross-linking, representing a range of gel content, swelling ability, elastic and viscous behavior were studied, as well as solutions of native HA of different molecular weights. T-1, T-2 and D were measured for several concentrations of HA and as a function of temperature. D and T-1 showed a weak concentration dependence, but did not differ between the hydrogels. T-2, dominated by chemical exchange between water protons and exchangeable protons of HA, varied significantly between the different hydrogels and the temperature profiles changed dramatically between different concentrations.

Keywords

Hyaluronic acid; Hydrogel; NMR; Water; Relaxation; Diffusion

Published in

Carbohydrate Polymers
2020, Volume: 248, article number: 116768
Publisher: ELSEVIER SCI LTD

SLU Authors

• Wende, Frida

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Xue, Yan

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Nestor, Gustav

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Sandström, Corine

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Polymer Chemistry


Publication identifier

DOI: https://doi.org/10.1016/j.carbpol.2020.116768

Permanent link to this page (URI)

https://res.slu.se/id/publ/108123