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Research article - Peer-reviewed, 2020

Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings

Alallaq, Sanaria; Ranjan, Alok; Brunoni, Federica; Novak, Ondrej; Lakehal, Abdellah; Bellini, Catherine

Abstract

Vegetative propagation relies on the capacity of plants to regeneratede novoadventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors, such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular components mediating light cues during AR initiation (ARI) remain largely elusive. Here, we explored the role of red light (RL) on ARI in de-rooted Norway spruce seedlings. We combined investigation of hormone metabolism and gene expression analysis to identify potential signaling pathways. We also performed extensive anatomical characterization to investigate ARI at the cellular level. We showed that in contrast to white light, red light promoted ARI likely by reducing jasmonate (JA) and JA-isoleucine biosynthesis and repressing the accumulation of isopentyl-adenine-type cytokinins. We demonstrated that exogenously applied JA and/or CK inhibit ARI in a dose-dependent manner and found that they possibly act in the same pathway. The negative effect of JA on ARI was confirmed at the histological level. We showed that JA represses the early events of ARI. In conclusion, RL promotes ARI by repressing the accumulation of the wound-induced phytohormones JA and CK.

Keywords

adventitious roots; conifers; Picea abies; auxin; cytokinins; jasmonate; red light; root development

Published in

Frontiers in Plant Science
2020, volume: 11, article number: 586140
Publisher: FRONTIERS MEDIA SA

Authors' information

Alallaq, Sanaria
Umea University
Ranjan, Alok
Umea University
Brunoni, Federica
Umea University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Palacký University Olomouc
Institute of Experimental Botany ASCR
Lakehal, Abdellah
Umea University
Bellini, Catherine
Umea University
Bellini, Catherine
Universite Paris Saclay

UKÄ Subject classification

Botany

Publication Identifiers

DOI: https://doi.org/10.3389/fpls.2020.586140

URI (permanent link to this page)

https://res.slu.se/id/publ/108219