Extenders for alpaca epididymal spermatozoa: Comparison of INRA96 and andromed

Abstract

Artificial insemination would be a useful technique for alpaca breeders to use as an aid to breeding to increase fleece quality. The technique, however, is not well developed in alpacas, partly because of the viscous nature of their seminal plasma. Castration conducted for husbandry purposes can provide a source of epididymal spermatozoa to test semen extenders or handling regimens, thus circumventing the problem of the viscous ejaculate. In this experiment, two semen extenders (Andromed and INRA96) developed for other species (bovine and equine, respectively) were tested with alpaca spermatozoa derived from the cauda epididymis. Sperm total motility (mean ± SEM A: 29.1 ± 4.8 % compared with I: 35.4 ± 4.8 %; NS), membrane integrity (A: 58 ± 9% compared with I: 56 ± 9%; NS) and acrosome integrity (A: 65 ± 7% compared with I: 54 ± 7%; NS) were not different between the two extenders. Progressive motility with use of INRA96 was greater after incubating for 30 min than after incubating for 10 min (35 ± 4% vs. 12 ± 4%, respectively; P = 0.03). In conclusion, viable epididymal spermatozoa could be extracted from the castrated organs after overnight transport. There were no differences in sperm quality between the two extenders; therefore, it appears that either extender could be used for alpaca spermatozoa. These results could help in the development of a technique for artificial insemination in alpacas.

Keywords

Vicugna pacos; Camelid; Sperm handling; Sperm quality

Published in

Animal Reproduction Science
2020, volume: 223, article number: 106629

SLU Authors

• Al-Essawe, Essraa M

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Al-Nahrain University

• Abraham, Maria Celina

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Kunkitti, Panisara

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Khon Kaen University

• Axner, Eva

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Båge, Renee

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Morrell, Jane

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics and Breeding in Agricultural Sciences
Clinical Science

Publication identifier

• DOI: https://doi.org/10.1016/j.anireprosci.2020.106629

Permanent link to this page (URI)

https://res.slu.se/id/publ/108267