Skip to main content
Research article - Peer-reviewed, 2020

Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species

Vetukuri, Ramesh R.; Kalyandurg, Pruthvi B.; Saripella, Ganapathi Varma; Sen, Diya; Gil, Jose Fernando; Lukhovitskaya, Nina, I; Grenville-Briggs, Laura J.; Savenkov, Eugene, I

Abstract

Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5 '-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.

Published in

Archives of Virology
2020, volume: 165, number: 12, pages: 2953-2959
Publisher: SPRINGER WIEN

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Breeding
Sen, Diya
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Biology
Lukhovitskaya, Nina
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Biology

UKÄ Subject classification

Microbiology

Publication Identifiers

DOI: https://doi.org/10.1007/s00705-020-04832-y

URI (permanent link to this page)

https://res.slu.se/id/publ/108415