Abstract

Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5 '-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.

Published in

Archives of Virology
2020, Volume: 165, number: 12, pages: 2953-2959
Publisher: SPRINGER WIEN

SLU Authors

• Vetukuri, Ramesh

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Kalyandurg, Pruthvi Balachandra

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Saripella, Ganapathi Varma

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Sen, Diya

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Gil, José Fernando

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Lukhovitskaya, Nina

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Grenville-Briggs Didymus, Laura

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Savenkov, Eugene

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Microbiology


Publication identifier

DOI: https://doi.org/10.1007/s00705-020-04832-y

Permanent link to this page (URI)

https://res.slu.se/id/publ/108415