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Research article2006Peer reviewed

Effects of mating on plasma concentrations of testosterone, cortisol, oestrone sulphate and 15-ketodihydro-PGF2 alpha in stallions

Villani M, Cairoli F, Kindahl H, Galeati G, Faustini M, Carluccio A, Veronesi MC


Very little information is available regarding the physiological mechanisms involved in the normal sexual activity in the stallion and, in particular, the endocrine control of reproduction is still not clearly understood. This experiment was designed to determine the short-term effect of sexual stimulation on plasma concentrations of testosterone, cortisol, oestrone sulphate and 15-ketodihydro-PGF2, in stallions. Semen samples were collected from 10 lighthorse stallions of proven fertility using a Missouri model artificial vagina. At the same time, blood samples were collected from the jugular vein with heparinized tubes, 20 and 10 min before oestrous mare exposure, at exposure and 10, 20, 30 min after dismounting. Testosterone concentrations showed a sharp rise 10 min after mating (p < 0.001), reached a plateau, and then showed a further increase 30 min after mating (p < 0.001). Cortisol concentrations increased 10 min after mating (p < 0.001) and remained at high levels in the subsequent samples taken. A peak of oestrone sulphate was observed 10 min after mating (p < 0.001). 15-Ketodihydro-PGF(2 alpha), concentrations. decreased rapidly at the moment of the exposure of the stallions to an oestrous mare (p < 0.05), returned to premating concentrations and then decreased again 30 min after mating (p < 0.05)

Published in

Reproduction in Domestic Animals
2006, Volume: 41, number: 6, pages: 544-548

    UKÄ Subject classification

    Animal and Dairy Science
    Veterinary Science

    Publication identifier


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