Hevea brasiliensis coniferaldehyde-5-hydroxylase (HbCAld5H) regulates xylogenesis, structure and lignin chemistry of xylem cell wall in Nicotiana tabacum

Abstract

Key message The HbCAld5H1 gene cloned from Hevea brasiliensis regulates the cambial activity, xylem differentiation, syringyl-guaiacyl ratio, secondary wall structure, lignification pattern and xylan distribution in xylem fibres of transgenic tobacco plants. Molecular characterization of lignin biosynthesis gene coniferaldehyde-5-hydroxylase (CAld5H)fromHevea brasiliensisand its functional validation was performed. Both sense and antisense constructs ofHbCAld5H1gene were introduced into tobacco throughAgrobacterium-mediated genetic transformation for over expression and down-regulation of this key enzyme to understand its role affecting structural and cell wall chemistry. The anatomical studies of transgenic tobacco plants revealed the increase of cambial activity leading to xylogenesis in sense lines and considerable reduction in antisense lines. The ultra-structural studies showed that the thickness of secondary wall (S2 layer) of fibre had been decreased with non-homogenous lignin distribution in antisense lines, while sense lines showed an increase in S2 layer thickness. Maule color reaction revealed that syringyl lignin distribution in the xylem elements was increased in sense and decreased in antisense lines. The immunoelectron microscopy revealed a reduction in LM 10 and LM 11 labelling in the secondary wall of antisense tobacco lines. Biochemical studies showed a radical increase in syringyl lignin in sense lines without any significant change in total lignin content, while S/G ratio decreased considerably in antisense lines. Our results suggest thatCAld5Hgene plays an important role in xylogenesis stages such as cambial cell division, secondary wall thickness, xylan and syringyl lignin distribution in tobacco. Therefore,CAld5Hgene could be considered as a promising target for lignin modification essential for timber quality improvement in rubber.

Keywords

Coniferaldehyde-5-hydroxylase; Hevea brasiliensis; Xylogenesis; Cell wall structure; Lignin; S; G ratio; Xylans

Published in

Plant Cell Reports
2021, volume: 40, number: 1, pages: 127-142
Publisher: SPRINGER

SLU Authors

• Pramod, Sivan

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • Rubber Research Institute of India

UKÄ Subject classification

Plant Biotechnology

Publication identifier

• DOI: https://doi.org/10.1007/s00299-020-02619-8

Permanent link to this page (URI)

https://res.slu.se/id/publ/108650