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Research article - Peer-reviewed, 2020

Diversity and Abundance of Microbial Communities in UASB Reactors during Methane Production from Hydrolyzed Wheat Straw and Lucerne

Liu, Tong; Schnurer, Anna; Bjorkmalm, Johanna; Willquist, Karin; Kreuger, Emma


The use of straw for biofuel production is encouraged by the European Union. A previous study showed the feasibility of producing biomethane in upflow anaerobic sludge blanket (UASB) reactors using hydrolyzed, steam-pretreated wheat straw, before and after dark fermentation withCaldicellulosiruptor saccharolyticus, and lucerne. This study provides information on overall microbial community development in those UASB processes and changes related to acidification. The bacterial and archaeal community in granular samples was analyzed using high-throughput amplicon sequencing. Anaerobic digestion model no. 1 (ADM1) was used to predict the abundance of microbial functional groups. The sequencing results showed decreased richness and diversity in the microbial community, and decreased relative abundance of bacteria in relation to archaea, after process acidification. Canonical correspondence analysis showed significant negative correlations between the concentration of organic acids and three phyla, and positive correlations with seven phyla. Organic loading rate and total COD fed also showed significant correlations with microbial community structure, which changed over time. ADM1 predicted a decrease in acetate degraders after a decrease to pH <= 6.5. Acidification had a sustained effect on the microbial community and process performance.


wheat straw hydrolysate; microbial community; next-generation amplicon sequencing; UASB; ADM1; process acidification; methane production; VFA

Published in

2020, volume: 8, number: 9, article number: 1394
Publisher: MDPI

Authors' information

Swedish University of Agricultural Sciences, Department of Molecular Sciences
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Björkmalm, Johanna
RISE Research Institutes of Sweden
Willquist, Karin
RISE Research Institutes of Sweden
Kreuger, Emma
Lund University

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