Tomato protoplasts as cell target for ribonucleoprotein (RNP)-mediated multiplexed genome editing

Abstract

The possibility to produce plants edited in multiple genes by means of DNA-free approaches opens new perspectives for breeding purposes and acceptance of resultant genotypes. In this work, we have explored the polyethylene glycol (PEG)-mediated delivery of ribonucleoproteins (RNPs) in tomato protoplasts using a multiplexing approach (i.e. two genes targeted simultaneously using two sgRNAs per gene) for the first time. We have analysed the editing outcome in fully developed green calli and demonstrated that tomato protoplasts are a valid cell target for RNP-mediated multiplexed genome editing with high efficiency.Key message RNP could be applied with high efficiency in a multiplexing genome editing approach in tomato protoplasts.

Keywords

Tomato; Ribonucleoprotein; Genome editing; Protoplast

Published in

Plant Cell, Tissue and Organ Culture
2021, Volume: 144, number: 2, pages: 463-467 Publisher: SPRINGER

SLU Authors

• Andersson, Mariette

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Hofvander, Per

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics and Breeding


Publication identifier

DOI: https://doi.org/10.1007/s11240-020-01954-8

Permanent link to this page (URI)

https://res.slu.se/id/publ/108725