Visualizing and measuring single locus dynamics in Arabidopsis thaliana

Abstract

In eukaryotes, DNA is packed into an incredibly complex structure called chromatin. Although chromatin
was often considered as a static entity, it is now clear that chromatin proteins and the chromatin fiber itself
are in fact very dynamic. For instance, the packaging of the DNA into the nucleus requires an extraordinary
degree of compaction but this should be achieved without compromising the accessibility to the transcription
machinery and other nuclear processes. Approaches such as gene tagging have been established for
living cells in order to detect, track, and analyze the mobility of single loci. In this chapter, we provide an
experimental protocol for performing locus tracking in Arabidopsis thaliana roots and for characterizing
locus mobility behavior via a Mean Square Displacement analysis.

Published in

Methods in Molecular Biology
2021, Volume: 2200, number: volume 2200, pages: 213-224 Title: Arabidopsis protocols
ISBN: 978-1-0716-0879-1, eISBN: 978-1-0716-0880-7Publisher: Springer Nature

SLU Authors

• Meschichi, Anis

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Rosa, Stefanie

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1007/978-1-0716-0880-7_10

Permanent link to this page (URI)

https://res.slu.se/id/publ/108799