Skip to main content
Research article - Peer-reviewed, 2020

Genetic control of tracheid properties in Norway spruce wood

Baison, John; Zhou, Linghua; Forsberg, Nils; Mörling, Tommy; Grahn, Thomas; Olsson, Lars; Karlsson, Bo; Wu, Harry; Mellerowicz, Ewa; Lundqvist, Sven-Olof; Garcia Gil, Rosario

Abstract

Through the use of genome-wide association studies (GWAS) mapping it is possible to establish the genetic basis of phenotypic trait variation. Our GWAS study presents the first such effort in Norway spruce (Picea abies (L). Karst.) for the traits related to wood tracheid characteristics. The study employed an exome capture genotyping approach that generated 178 101 Single Nucleotide Polymorphisms (SNPs) from 40 018 probes within a population of 517 Norway spruce mother trees. We applied a least absolute shrinkage and selection operator (LASSO) based association mapping method using a functional multi-locus mapping approach, with a stability selection probability method as the hypothesis testing approach to determine significant Quantitative Trait Loci (QTLs). The analysis has provided 30 significant associations, the majority of which show specific expression in wood-forming tissues or high ubiquitous expression, potentially controlling tracheids dimensions, their cell wall thickness and microfibril angle. Among the most promising candidates based on our results and prior information for other species are: Picea abies BIG GRAIN 2 (PabBG2) with a predicted function in auxin transport and sensitivity, and MA_373300g0010 encoding a protein similar to wall-associated receptor kinases, which were both associated with cell wall thickness. The results demonstrate feasibility of GWAS to identify novel candidate genes controlling industrially-relevant tracheid traits in Norway spruce.

Published in

Scientific Reports
2020, volume: 10, number: 1, article number: 18089

Authors' information

Baison, John
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Zhou, Linghua
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Forsberg, Nils
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Ecology and Management
Grahn, Thomas
RISE Bioeconomy
Olsson, Lars
RISE Bioeconomy
Karlsson, Bo
Skogforsk
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Lundqvist, Sven-Olof
RISE Bioeconomy
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Forest Science

Publication Identifiers

DOI: https://doi.org/10.1038/s41598-020-72586-3

URI (permanent link to this page)

https://res.slu.se/id/publ/109055