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Research article - Peer-reviewed, 2020

Country-Wide qPCR Based Assessment of Plasmodiophora brassicae Spread in Agricultural Soils and Recommendations for the Cultivation of Brassicaceae Crops in Poland

Czubatka-Bieńkowska, Anna; Kaczmarek, Joanna; Marzec-Schmidt, Katarzyna; Nieróbca, Anna; Czajka, Agnieszka; Jędryczka, Małgorzata


Clubroot is a damaging disease of oilseed rape and vegetable brassicas worldwide, caused by the soil-borne protist Plasmodiophora brassicae Wor. Due to the long life of resting spores, the assessment of the pathogen abundance in agricultural fields can serve as a guideline for disease control at the country-wide level or the regional scale. Between 2013 and 2019, we collected 431 soil samples from fields cultivated with Brassicaceae crops throughout 16 provinces of Poland. The samples were subjected to qPCR based analysis of P. brassicae DNA concentration. From these data, the spore loads and gene copies g−1 soil were calculated and used to produce an assessment of the current clubroot risk potential at a country-wide and regional scale. The country-wide map, showing the spread of the pathogen in agricultural soils, was made using ArcGis software package implementing the interpolation with the Inverse Distance Weight method. The calculation of gene copies specific to P. brassicae helped to formulate the recommendations for farmers in respect to the cultivation guidelines. It showed a high risk of yield losses in defined regions of north, south-west and central Poland and an urgent need to undertake intensive preventative measures.


clubroot; pedology; soil infestation; soil mapping

Published in

2020, volume: 9, number: 12, article number: 1070

Authors' information

Czubatka-Bieńkowska, Anna
Research Institute of Horticulture
Kaczmarek, Joanna
Polish Academy of Sciences (PAN)
Swedish University of Agricultural Sciences, Department of Soil and Environment
Nieróbca, Anna
Pope John Paul II State School of Higher Education in Biała Podlaska
Czajka, Agnieszka
Research Institute of Horticulture
Jędryczka, Małgorzata
Polish Academy of Sciences (PAN)

Associated SLU-program

SLU Network Plant Protection

UKÄ Subject classification

Agricultural Science
Geosciences, Multidisciplinary

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