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Research article - Peer-reviewed, 2021

Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress

Liu, Shujing; de Jonge, Jennifer; Trejo-Arellano, Minerva S.; Santos-Gonzalez, Juan; Kohler, Claudia; Hennig, Lars

Abstract

Heat-stressed Arabidopsis plants release heterochromatin-associated transposable element (TE) silencing, yet it is not accompanied by major reductions of epigenetic repressive modifications. In this study, we explored the functional role of histone H1 in repressing heterochromatic TEs in response to heat stress.We generated and analyzed RNA and bisulfite-sequencing data of wild-type and h1 mutant seedlings before and after heat stress.Loss of H1 caused activation of pericentromeric Gypsy elements upon heat treatment, despite these elements remaining highly methylated. By contrast, nonpericentromeric Copia elements became activated concomitantly with loss of DNA methylation. The same Copia elements became activated in heat-treated chromomethylase 2 (cmt2) mutants, indicating that H1 represses Copia elements through maintaining DNA methylation under heat.We discovered that H1 is required for TE repression in response to heat stress, but its functional role differs depending on TE location. Strikingly, H1-deficient plants treated with the DNA methyltransferase inhibitor zebularine were highly tolerant to heat stress, suggesting that both H1 and DNA methylation redundantly suppress the plant response to heat stress.

Keywords

Arabidopsis thaliana; CMT2; DNA methylation; H1; heat stress; transposable element

Published in

New Phytologist
2021, volume: 229, number: 4, pages: 2238-2250
Publisher: WILEY

Authors' information

Liu, Shujing
Swedish University of Agricultural Sciences, Department of Plant Biology
De Jonge, Jennifer (De Jonge, Jennifer)
Swedish University of Agricultural Sciences, Department of Plant Biology
Trejo-Arellano, Minerva S. (Trejo-Arellano, Minerva S.)
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Biology
Hennig, Lars (Hennig, Lars)
Swedish University of Agricultural Sciences, Department of Plant Biology

UKÄ Subject classification

Botany

Publication Identifiers

DOI: https://doi.org/10.1111/nph.17018

URI (permanent link to this page)

https://res.slu.se/id/publ/109340