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Research article2020Peer reviewedOpen access

Arabidopsis RING-type E3 ubiquitin ligase XBAT35.2 promotes proteasome-dependent degradation of ACD11 to attenuate abiotic stress tolerance

Li, Qiaomu; Serio, Renata J.; Schofield, Andrew; Liu, Hongxia; Rasmussen, Sheena R.; Hofius, Daniel; Stone, Sophia L.

Abstract

Plants employ multiple mechanisms to cope with a constantly changing and challenging environment, including using the ubiquitin proteasome system (UPS) to alter their proteome to assist in initiating, modulating and terminating responses to stress. We previously reported that the ubiquitin ligase XBAT35.2 mediates the proteasome-dependent degradation of Accelerated Cell Death 11 (ACD11) to promote pathogen defense. Here, we demonstrate roles for XBAT35.2 and ACD11 in abiotic stress tolerance. As seen in response to pathogen infection, abiotic stress stabilizes XBAT35.2 and the abundance of ACD11 rose consistently with increasing concentrations of abscisic acid (ABA) and salt. Surprisingly, exposure to ABA and salt increased the stability of ACD11, and the overexpression of ACD11 improves plant survival of salt and drought stress, suggesting a role for ACD11 in promoting tolerance. Prolonged exposure to high concentrations of ABA or salt resulted in ubiquitination and the proteasome-dependent degradation of ACD11, however. The stress-induced turnover of ACD11 requires XBAT35.2, as degradation is slowed in the absence of the E3 ubiquitin ligase. Consistent with XBAT35.2 mediating the proteasome-dependent degradation of ACD11, the loss of E3 ubiquitin ligase function enhances the tolerance of salt and drought stress, whereas overexpression increases sensitivity. A model is presented where, upon the perception of abiotic stress, ACD11 abundance increases to promote tolerance. Meanwhile, XBAT35.2 accumulates and in turn promotes the degradation of ACD11 to attenuate the stress response. The results characterize XBAT35.2 as an E3 ubiquitin ligase with opposing roles in abiotic and biotic stress.

Keywords

abiotic stress tolerance; abscisic acid (ABA); ubiquitin proteasome system (UPS); RING‐type E3 ubiquitin ligase; XBAT35 (for XB3 ortholog 5 in Arabidopsis thaliana); regulated protein degradation

Published in

Plant Journal
2020, Volume: 104, number: 6, pages: 1712-1723 Publisher: WILEY

    Associated SLU-program

    SLU Plant Protection Network

    UKÄ Subject classification

    Botany

    Publication identifier

    DOI: https://doi.org/10.1111/tpj.15032

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/109342