Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine

Abstract

A new Lactobacillus species from pig small intestine has been identified. In an attempt to isolate Lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. A number of isolates were obtained and primary characterization showed that they were Cram-positive, catalase-negative, non-spore-forming, non-motile rods. Growth occurred at 45 degrees C but not at 15 degrees C and the DNA G+C content was 46 mol%. Cell wall analysis together with DNA-DNA hybridization and analysis of the 165 rRNA sequence revealed that the new isolates represent a previously undescribed Lactobacillus species closely related to L. reuteri. Lactobacillus fermentum and Lactobacillus pontis. The name Lactobacillus mucosae is proposed for this species and the type strain is S32(T).

Keywords

Lactobacillus mucosae; new species; mucus binding

Published in

International Journal of Systematic and Evolutionary Microbiology
2000, Volume: 50, pages: 251-258

SLU Authors

• Roos, Stefan

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Jonsson, Hans

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Microbiology


Publication identifier

DOI: https://doi.org/10.1099/00207713-50-1-251

Permanent link to this page (URI)

https://res.slu.se/id/publ/109408