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Research article - Peer-reviewed, 2021

Lead contamination alters enzyme activities and microbial composition in the rhizosphere soil of the hyperaccumulator Pogonatherum crinitum

Hou, Xiao-long; Han, Hang; Tigabu, Mulualem; Li, Qi-yan; Li, Zong-xun; Zhu, Chen-lu; Huang, Si-qi; Cai, Li-ping; Liu, Ai-qin

Abstract

Pogonatherum crinitum is a promising lead (Pb) hyperaccumulator; however, the effects of Pb contamination on P. crinitum rhizosphere soil enzymatic activities and microbial composition remain largely unexplored. Thus, an indoor experiment was conducted by cultivating P. crinitum seedlings and exposing them to four Pb concentrations (0, 1,000, 2000 and 3000 mg/kg Pb). Protease, urease, acid phosphatase and invertase activities were determined using standard methods while soil bacterial composition was determined by 16 S rDNA sequencing. The results showed that rhizosphere soil acid phosphatase activity significantly increased with increasing Pb concentration, while urease activity was significantly greater in rhizosphere soil contaminated with 1000 and 2000 mg/kg than in the control. There was a clear shift in bacterial composition during phytoremediation by P. crinitum. Compared to the control, Bacteroidetes was more abundant in all Pb-contaminated soils, Actinobacteria was more abundant in 1000 mg/kg Pb-treated soil, and Firmicutes was more abundant in 3000 mg/kg Pb-treated soil. Positive correlations were observed between dominant bacterial phyla and soil enzyme activities. Metabolic pathways, such as ABC transporter, quinine reductase, and ATP-binding protein were significantly increased in rhizosphere soil bacteria with Pb contamination. In conclusion, Pb contamination differentially influenced the activities of rhizosphere soil enzymes, specifically increasing acid phosphatase and urease activities, and alters the dominance of soil bacteria through up-regulation of genes related to some metabolic pathways. The strong correlations between dominant bacterial phyla and enzymatic activities suggest synergetic effects on the growth of P. crinitum during Pb contamination.

Keywords

16S rDNA Bacteroidetes; Proteobacteria; protease; urease; Acid phosphatase

Published in

Ecotoxicology and Environmental Safety
2021, volume: 207, article number: 111308
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

Authors' information

Hou, Xiao-long
Fujian Agriculture and Forestry University
Han, Hang
Fujian Agriculture and Forestry University
Fujian Agriculture and Forestry University
Swedish University of Agricultural Sciences, Southern Swedish Forest Research Centre
Li, Qi-yan
Fujian Agriculture and Forestry University
Li, Zong-xun
Fujian Agriculture and Forestry University
Zhu, Chen-lu
Fujian Agriculture and Forestry University
Huang, Si-qi
Fujian Agriculture and Forestry University
Cai, Li-ping
Fujian Agriculture and Forestry University
Liu, Ai-qin
Fujian Agriculture and Forestry University

UKÄ Subject classification

Environmental Sciences

Publication Identifiers

DOI: https://doi.org/10.1016/j.ecoenv.2020.111308

URI (permanent link to this page)

https://res.slu.se/id/publ/109726