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Research article2002Peer reviewed

KNOTTED1-like homeobox genes of a gymnosperm, Norway spruce, expressed during somatic embryogenesis

Hjortswang, HI; Larsson, AS; Bharathan, G; Bozhkov, PV; von Arnold, S; Vahala, T

Abstract

Two Norway spruce (Picea abies (L.) Karst.) genes belonging to class I of the KNOTTED1-like homeobox (KNOX) genes, HBK2 and HBK3, were cloned with PCR-based methods. The expression of these and a previously characterised related gene, HBK1, in different organs and during somatic embryogenesis was studied with RTPCR. Transcripts of all three genes were detected in stems, roots and in cone buds, but not in needles. HBK1 and HBK3 are expressed throughout development in a normal cell line with embryogenic potential and in a cell line unable to form somatic embryos. HBK2 is expressed in the normal cell line, but not in the developmentally arrested cell line. This suggests that the HBK2 gene is involved in the somatic embryo development. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

Keywords

development; KNOX genes; Picea abies; somatic embryogenesis

Published in

Plant Physiology and Biochemistry
2002, Volume: 40, number: 10, article number: PII S0981-9428(02)01445-6

      SLU Authors

    • Bozhkov, Peter

      • Department of Forest Genetics, Swedish University of Agricultural Sciences

      • Von Arnold, Sara

        • Department of Forest Genetics, Swedish University of Agricultural Sciences

        • Vahala, Tiina

          • Department of Forest Genetics, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Forest Science

        Publication identifier

        DOI: https://doi.org/10.1016/S0981-9428(02)01445-6

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/109915