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Research article - Peer-reviewed, 2020

Reaction Wood Anatomical Traits and Hormonal Profiles in Poplar Bent Stem and Root

De Zio, Elena; Montagnoli, Antonio; Karady, Michal; Terzaghi, Mattia; Sferra, Gabriella; Antoniadi, Ioanna; Scippa, Gabriella S.; Ljung, Karin; Chiatante, Donato; Trupiano, Dalila


Reaction wood (RW) formation is an innate physiological response of woody plants to counteract mechanical constraints in nature, reinforce structure and redirect growth toward the vertical direction. Differences and/or similarities between stem and root response to mechanical constraints remain almost unknown especially in relation to phytohormones distribution and RW characteristics. Thus, Populus nigra stem and root subjected to static non-destructive mid-term bending treatment were analyzed. The distribution of tension and compression forces was firstly modeled along the main bent stem and root axis; then, anatomical features, chemical composition, and a complete auxin and cytokinin metabolite profiles of the stretched convex and compressed concave side of three different bent stem and root sectors were analyzed. The results showed that in bent stems RW was produced on the upper stretched convex side whereas in bent roots it was produced on the lower compressed concave side. Anatomical features and chemical analysis showed that bent stem RW was characterized by a low number of vessel, poor lignification, and high carbohydrate, and thus gelatinous layer in fiber cell wall. Conversely, in bent root, RW was characterized by high vessel number and area, without any significant variation in carbohydrate and lignin content. An antagonistic interaction of auxins and different cytokinin forms/conjugates seems to regulate critical aspects of RW formation/development in stem and root to facilitate upward/downward organ bending. The observed differences between the response stem and root to bending highlight how hormonal signaling is highly organ-dependent.


bending stress; auxins; cytokinins; metabolite profiling; UHPLC-MS; MS

Published in

Frontiers in Plant Science
2020, volume: 11, article number: 590985

Authors' information

De Zio, Elena
University of Molise
Montagnoli, Antonio
University of Insubria
Karady, Michal
Institute of Experimental Botany of the Czech Academy of Sciences
Terzaghi, Mattia
University of Salerno
Sferra, Gabriella
University of Molise
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Scippa, Gabriella S.
University of Molise
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Chiatante, Donato
University of Insubria
Trupiano, Dalila
University of Molise

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