Abstract

The evolution of pathogens in the changing climate raises new challenges for wheat production. Yellow rust is one of the major wheat diseases worldwide, leading to an increased use of fungicides to prevent significant yield losses. The enhancement of the resistance potential of wheat cultivars is a necessary and environmentally friendly solution for sustainable wheat production. In this study, we aimed to identify the differentially expressed genes induced upon yellow rust infection in the field. Reference and de novo based transcriptome analysis was performed among the resistant and susceptible lines of a bi-parental population to study the global transcriptome changes in contrasting wheat genotypes. Based on the analysis, the de novo transcriptome analysis approach was found to be more supportive for field studies. Expression profiles, gene ontology, KEGG pathway analysis and enrichment studies indicated the relation between differentially expressed genes of wheat and yellow rust infection. The h0igh expression of genes related to non-race specific resistance along with pathogen-specific resistance might be a reason for the better resistance ability of a resistant wheat genotype in the field. The targeted metagenomic analysis of wheat samples revealed that Puccinia striiformis tritici was the most dominant pathogen along with other pathogens on the collected leaf material and validating the disease scoring carried out in the field and transcriptomics analyses.

Keywords

wheat; yellow rust; disease resistance; transcriptomics; differential gene expression

Published in

Agronomy
2020, Volume: 10, number: 12, article number: 1888
Publisher: MDPI

SLU Authors

• Kushwaha, Sandeep Kumar

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    
  • National Institute of Animal Biotechnology

• Vetukuri, Ramesh

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Odilbekov, Firuz

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

• Chawade, Aakash

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    

Associated SLU-program

AMR: Fungus


Sustainable Development Goals

Ensure sustainable consumption and production patterns


UKÄ Subject classification

Agricultural Science


Publication identifier

DOI: https://doi.org/10.3390/agronomy10121888

Permanent link to this page (URI)

https://res.slu.se/id/publ/110209