Abstract

To increase the knowledge of microbial adaptation for high degradation ability under ammonia stressed conditions, the metabolic performance and the microbial community in a full-scale chicken manure anaerobic digestion (AD) plant, operated over a 10-year, were investigated. The methanogenic pathway employing labeled acetate (2-C-13), aceticlastic methanogenesis activity, and the Illumina sequencing of microbiome structure at the plant were analyzed. The obtained results show that this active plant achieved a methane yield of 310 +/- 43 mL/g-volatile solids (VS), high stability under an ammonium-N level of 6.2 +/- 0.1 g/L, and an organic loading rate of 2.5 g-VS/(L.d). The extremely long adaptation developed the unique microbe communities, and the hydrogentrophic methanogens Methanobrevibacter (76%) and Methanoculleus (18%) were dominant in the main digester. The acetoclastic methanogenic pathway reached 42%, contributing to the deep degradation. The current study thus may lay the foundation for illustrating the successful full-scale AD of nitrogen-rich materials under stressed operating conditions. (c) 2020 Elsevier Ltd. All rights reserved.

Keywords

Anaerobic digestion; Full-scale biogas plant; Microbial acclimation; Methanogenic pathway; Microbial communities; Chicken manure

Published in

Renewable Energy
2021, Volume: 167, pages: 644-651
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

SLU Authors

• Westerholm, Maria

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

Sustainable Development Goals

Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation


UKÄ Subject classification

Microbiology
Bioenergy


Publication identifier

DOI: https://doi.org/10.1016/j.renene.2020.11.133

Permanent link to this page (URI)

https://res.slu.se/id/publ/110504