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Research article2001Peer reviewed

A chiral sex pheromone system in the pea midge, Contarinia pisi

Hillbur, Y; Bengtsson, M; Lofqvist, J; Biddle, A; Pillon, O; Plass, E; Francke, W; Hallberg, E

Abstract

The sex pheromone of the pea midge consists of 2-acetoxytridecane, (2S,11S)-diacetoxytridecane and (2S,12S)-diacetoxytridecane. The responses of male pea midges to the corresponding stereoisomers of (2S,11S)-diacetoxytridecane and (2S, I 2S)-diacetoxytridecane were tested in field trapping experiments and by electroantennographic recordings. When added at 20% of the pheromone component to the sex pheromone blend. the (2S,11R)- and (2R,11S)stereoisomers of (2S, I IS)-diacetoxytridecane, were shown to have a strong inhibitory effect on male attraction in the field. At the same dose, (2R,11R)diacetoxytridecane, (2R,12R)-diacetoxytridecane, and meso-2,12-diacetoxytridecane, did not have a significant effect on male behavior. It was also shown that substitution of either (2S, 11S)-diacetoxytridecane or (2S, I 2S)-diacetoxytridecane with the related stereoisomers reduced trap catches to the level of blank traps. The electroantennographic recordings showed similar dose-response curves for the pheromone components and the stereoisomers shown to have an inhibitory effect. It seems likely that male antennae have receptors for both pheromone components and for inhibitory stereoisomers. Scanning electron microscopy and transmission electron microscopy of the antennae revealed three types of sensilla involved in chemoreception: sensilla circumfila, sensilla trichodea. and sensilla coeloconica. The sensilla circumfila and trichodea are both innervated by two sensory cells, whereas the sensilla coeloconica are innervated by four to five cells.

Keywords

Contarinia pisi; Cecidomyiidae; Diptera; sex pheromone; (2S, 11S)-diacetoxytridecane; (2S, 12S)-diacetoxytridecane; stereoisomers; inhibition; EAG recordings; antennal morphology

Published in

Journal of Chemical Ecology
2001, Volume: 27, number: 7, pages: 1391-1407

      SLU Authors

    • Hillbur, Ylva

      • Department of Crop Science, Swedish University of Agricultural Sciences

      • Bengtsson, Marie

        • Department of Crop Science, Swedish University of Agricultural Sciences

        • Löfqvist, Jan

          • Department of Crop Science, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Biochemistry and Molecular Biology

        Publication identifier

        DOI: https://doi.org/10.1023/A:1010317310027

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/110568