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Research article2021Peer reviewed

Detection of Porcine Cysticercosis in Meat Juice Samples from Infected Pigs

Maganira, Justine Daudi; Kidima, Winifrida; Mwita, Chacha John; Hoglund, Johan

Abstract

Background Seroprevalence of porcine cysticercosis has been generally studied using enzyme-linked immunosorbent assays (ELISA) detecting either antigens or antibodies in sera. However, serum is not always readily available. The objective of this study was to assess the diagnostic potential of meat juice in detecting porcine cysticercosis using a cysticercosis antibody ELISA. Methods Sera and meat juice samples from 13 different organs/tissues were collected from nine pigs naturally infected with cysticercosis and from six uninfected pigs reared under hygienic conditions. The sensitivity of the cysticercosis antibody ELISA in detecting porcine cysticercosis in meat juice samples was compared to that in serum samples from the same pigs. Results Using sera, cysticercosis was detected in all nine pigs harbouring cysticerci, but not in those reared under hygienic conditions. The sensitivity of the ELISA was highest in meat juice extracted from the diaphragm (100%), heart (89%) and neck muscle (78%) of the nine infected pigs, whereas it varied between 0 and 44% in the other samples. Conclusion To the best of our knowledge, this is the first study for T. solium cysticercosis serology to use meat juice. Our results show that meat juice from pig carcass organs or muscles is a promising diagnostic specimen for the detection of porcine cysticercosis. More studies including a large sample size of pigs with varying degrees of cysticercosis infection are needed to further prove this concept.

Keywords

Pigs; Taenia solium porcine cysticercosis; Meat juice; Serum; ELISA; Tanzania

Published in

Acta Parasitologica
2021, Volume: 66, number: 3, pages: 851-856
Publisher: SPRINGER INTERNATIONAL PUBLISHING AG

      SLU Authors

    • UKÄ Subject classification

      Clinical Science

      Publication identifier

      DOI: https://doi.org/10.1007/s11686-020-00295-y

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/110939