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Research article - Peer-reviewed, 2021

Freezing tolerance of seeds can explain differences in the distribution of two widespread mistletoe subspecies in Europe

Tikkanen, Olli-Pekka; Kilpelainen, Jouni; Mellado, Ana; Hamalainen, Aino; Hodar, Jose A.; Jaroszewicz, Bogdan; Luoto, Minna; Repo, Tapani; Rigling, Andreas; Wang, Ao; Li, Mai-He; Lehto, Tarja


The ability of plants to tolerate freezing limits their geographical distribution. Therefore, winter warming may shift a species' occurrence northwards and/or to higher altitudes. In Europe, the hemiparasitic vascular plant Viscum album (mistletoe) has two common and widespread subspecies: V. a. ssp. album and V. a. ssp. austriacum. The former has a more northern geographic distribution than the latter. Therefore we hypothesised that seeds of V. a. ssp. album are more tolerant to freezing than those of V. a. ssp. austriacum. From these two mistletoe subspecies V. a. ssp. austriacum is, in some managed forest areas, considered a novel threat to tree growth and forest health.Berries of V. a. ssp. album were collected from Sweden, Poland and Switzerland and berries of V. a. ssp. austriacum were collected from Poland, Switzerland and Spain. After storage at -3 degrees C, seeds were extracted from the pulp of berries and exposed to eight different temperatures between -8 degrees C and -30 degrees C, with the storage temperature serving as the control. After freezing treatments, germination of seeds was monitored. In addition, differential thermal analysis was used to measure freeze tolerance of seeds.The seeds of V. a. ssp. album tolerated lower temperatures than seeds of V. a. ssp. austriacum. The temperature at which 50% of seeds lost their ability to germinate (LT50) was -15 degrees C in V. a. ssp. austriacum and between -15 degrees C and - 19 degrees C in V. a. ssp. album. The results of differential thermal analysis to determine the freezing point of seeds supported these findings.The freezing tolerance of mistletoe seeds was relatively well coupled with the winter climate at the edge of their current geographic distribution. Based on our results, the warming of winters may eliminate the abiotic barrier that has thus far limited mistletoes' expansion, opening a window of opportunity for these parasites to increase their abundance and shift their distribution range towards higher latitudes and altitudes.Although mistletoes play important ecological roles in forest ecosystems, their recent increase has raised concern among forest managers, because they may cause a substantial reduction in tree growth in single species dominated stands. Increasing tree species diversity might be an effective method for limiting future mass infestations in homogeneous managed forests.


Climate change; Frost tolerance; Hemiparasite; Seed germination; Species distribution; Viscum album

Published in

Forest Ecology and Management
2021, volume: 482, article number: 118806
Publisher: ELSEVIER

Authors' information

Tikkanen, Olli-Pekka
University of Eastern Finland
Kilpelainen, Jouni
Natural Resources Institute Finland (Luke)
Mellado, Ana
University of Granada
Swedish University of Agricultural Sciences, Department of Ecology
Hodar, Jose A.
University of Granada
Jaroszewicz, Bogdan
University of Warsaw
Luoto, Minna
University of Eastern Finland
Repo, Tapani
Natural Resources Institute Finland (Luke)
Rigling, Andreas
ETH Zurich
Wang, Ao
ETH Zurich
Li, Mai-He
Swiss Federal Institute for Forest, Snow and Landscape Research (WSL)
Lehto, Tarja
University of Eastern Finland

UKÄ Subject classification

Forest Science

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