Abstract

Diamine and polyamine catabolism controls plant development, resistance to pathogens and stress responses. Diamine and polyamine oxidases control the catabolism of diamines and polyamines, respectively. Two major routes of di-/polyamine catabolism exist: the terminal and the interconverting. The in vitro activity of each route is assayed by the colorimetric or chemiluminescent determination of hydrogen peroxide produced by oxidation of di-/polyamine substrates. However, these assays fail to estimate activity of individual di-/polyamine oxidase isoenzymes. Herein, I describe an assay for the simultaneous in-gel determination of terminal and interconverting di-/polyamine oxidase isoenzyme activities.

Published in

Methods in Molecular Biology
2018, Volume: 1694, pages: 141-147

SLU Authors

• Moschou, Panagiotis Nikolaou

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

Associated SLU-program

SLU Plant Protection Network


UKÄ Subject classification

Agricultural Science


Publication identifier

DOI: https://doi.org/10.1007/978-1-4939-7398-9_14

Permanent link to this page (URI)

https://res.slu.se/id/publ/111043