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Research article - Peer-reviewed, 2021

EARLY BUD-BREAK 1 and EARLY BUD-BREAK 3 control resumption of poplar growth after winter dormancy

Azeez, Abdul; Zhao, Yiru Chen; Singh, Rajesh Kumar; Yordanov, Yordan S.; Dash, Madhumita; Miskolczi, Pal; Stojkovic, Katja; Strauss, Steve H.; Bhalerao, Rishikesh P.; Busov, Victor B.;

Abstract

Bud-break is an economically and environmentally important process in trees and shrubs from boreal and temperate latitudes, but its molecular mechanisms are poorly understood. Here, we show that two previously reported transcription factors, EARLY BUD BREAK 1 (EBB1) and SHORT VEGETATIVE PHASE-Like (SVL) directly interact to control bud-break. EBB1 is a positive regulator of bud-break, whereas SVL is a negative regulator of bud-break. EBB1 directly and negatively regulates SVL expression. We further report the identification and characterization of the EBB3 gene. EBB3 is a temperature-responsive, epigenetically-regulated, positive regulator of bud-break that provides a direct link to activation of the cell cycle during bud-break. EBB3 is an AP2/ERF transcription factor that positively and directly regulates CYCLIND3.1 gene. Our results reveal the architecture of a putative regulatory module that links temperature-mediated control of bud-break with activation of cell cycle. An AP2/ERF family gene EBB1 and a MADS-box gene SVL encode two regulators of poplar bud break. Here, the authors report another AP2/ERF transcription factor EBB3, which functions together with EBB1, SVL, and cell cycle progression promoter CYCD3.1 to regulate poplar bud break.

Published in

Nature Communications

2021, volume: 12, number: 1, article number: 1123
Publisher: NATURE RESEARCH

Authors' information

Michigan Technological University
Zhao, Yiru Chen
Michigan Technological University
Singh, Rajesh
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Singh, Rajesh
Council of Scientific and Industrial Research (CSIR)
Yordanov, Yordan S.
Michigan Technological University
Dash, Madhumita
Michigan Technological University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Strauss, Steve H.
Oregon State University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Busov, Victor B.
Michigan Technological University

UKÄ Subject classification

Forest Science

Publication Identifiers

DOI: https://doi.org/10.1038/s41467-021-21449-0

URI (permanent link to this page)

https://res.slu.se/id/publ/111114