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Research article - Peer-reviewed, 2021

Fungal X-Intrinsic Protein Aquaporin from Trichoderma atroviride: Structural and Functional Considerations

Ben Amira, Maroua; Faize, Mohamed; Karlsson, Magnus; Dubey, Mukesh; Frac, Magdalena; Panek, Jacek; Fumanal, Boris; Gousset-Dupont, Aurelie; Julien, Jean-Louis; Chaar, Hatem; Auguin, Daniel; Mom, Robin; Label, Philippe; Venisse, Jean-Stephane;

Abstract

The major intrinsic protein (MIP) superfamily is a key part of the fungal transmembrane transport network. It facilitates the transport of water and low molecular weight solutes across biomembranes. The fungal uncharacterized X-Intrinsic Protein (XIP) subfamily includes the full protein diversity of MIP. Their biological functions still remain fully hypothetical. The aim of this study is still to deepen the diversity and the structure of the XIP subfamily in light of the MIP counterparts-the aquaporins (AQPs) and aquaglyceroporins (AQGPs)-and to describe for the first time their function in the development, biomass accumulation, and mycoparasitic aptitudes of the fungal bioagent Trichoderma atroviride. The fungus-XIP Glade, with one member (TriatXIP), is one of the three clades of MIPs that make up the diversity of T. atroviride MIPs, along with the AQPs (three members) and the AQGPs (three members). TriatXIP resembles those of strict aquaporins, predicting water diffusion and possibly other small polar solutes due to particularly wider ar/R constriction with a Lysine substitution at the LE2 position. The XIP loss of function in Delta TriatXIP mutants slightly delays biomass accumulation but does not impact mycoparasitic activities. Delta TriatMIP forms colonies similar to wild type; however, the hyphae are slightly thinner and colonies produce rare chlamydospores in PDA and specific media, most of which are relatively small and exhibit abnormal morphologies. To better understand the molecular causes of these deviant phenotypes, a wide-metabolic survey of the ATriatXIPs demonstrates that the delayed growth kinetic, correlated to a decrease in respiration rate, is caused by perturbations in the pentose phosphate pathway. Furthermore, the null expression of the XIP gene strongly impacts the expression of four expressed MIP-encoding genes of T. atroviride, a plausible compensating effect which safeguards the physiological integrity and life cycle of the fungus. This paper offers an overview of the fungal XIP family in the biocontrol agent T. atroviride which will be useful for further functional analysis of this particular MIP subfamily in vegetative growth and the environmental stress response in fungi. Ultimately, these findings have implications for the ecophysiology of Trichoderma spp. in natural, agronomic, and industrial systems.

Keywords

aquaporin; uncharacterized X-Intrinsic proteins; Trichoderma atroviride; 3D modeling; chlamydospores; pentose phosphate pathway; stress responses

Published in

Biomolecules

2021, volume: 11, number: 2, article number: 338
Publisher: MDPI

Authors' information

Ben Amira, Maroua
Universite Clermont Auvergne (UCA)
Faize, Mohamed
University Chouaib Doukkali, El Jadida,
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Frac, Magdalena
Polish Academy of Sciences
Panek, Jacek
Polish Academy of Sciences
Fumanal, Boris
Universite Clermont Auvergne (UCA)
Gousset-Dupont, Aurelie
Universite Clermont Auvergne (UCA)
Julien, Jean-Louis
Universite Clermont Auvergne (UCA)
Chaar, Hatem
National Agronomic Institute of Tunisia (INAT)
Auguin, Daniel
Universite de Orleans
Mom, Robin
Universite Clermont Auvergne (UCA)
Label, Philippe
Universite Clermont Auvergne (UCA)
Venisse, Jean-Stephane
Universite Clermont Auvergne (UCA)

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.3390/biom11020338

URI (permanent link to this page)

https://res.slu.se/id/publ/111126