Kahlert, Maria
- Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
Research article2021Peer reviewedOpen access
Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life
Rimet, Frédéric; Aylagas, Eva; Borja, Angel; Bouchez, Agnes; Canino, Alexis; Chauvin, Christian; Chonova, Teofana; Ciampor, Fedor; Costa, Filipe O.; Ferrari , Benoit J. D.; Gastineau, Romain; Goulon, Chloé; Gugger, Muriel; Holzmann, Maria; Jahn, Regine; Kahlert, Maria; Kusber, Wolf-Henning; Laplace-Treyture, Christophe; Leese, Florian; Leliaert, Frederik;
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Abstract
DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.
Published in
Metabarcoding and Metagenomics
2021, Volume: 5, article number: e58056
SLU Authors
UKÄ Subject classification
Genetics
Publication identifier
DOI: https://doi.org/10.3897/mbmg.5.58056
Permanent link to this page (URI)
https://res.slu.se/id/publ/111140