Skip to main content
Research article - Peer-reviewed, 2021

Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

Rimet, Frédéric; Aylagas, Eva; Borja, Angel; Bouchez, Agnes; Canino, Alexis; Chauvin, Christian; Chonova, Teofana; Ciampor, Fedor; Costa, Filipe O.; Ferrari , Benoit J. D.; Gastineau, Romain; Goulon, Chloé; Gugger, Muriel; Holzmann, Maria; Jahn, Regine; Kahlert, Maria; Kusber, Wolf-Henning; Laplace-Treyture, Christophe; Leese, Florian; Leliaert, Frederik;
Show more authors


DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.

Published in

Metabarcoding and Metagenomics
2021, volume: 5, article number: e58056

Authors' information

Rimet, Frédéric
National Institute of Agricultural Research (INRA)
Aylagas, Eva
King Abdullah University of Science and Technology
Borja, Angel
Bouchez, Agnes
National Institute of Agricultural Research (INRA)
Canino, Alexis
National Institute of Agricultural Research (INRA)
Chauvin, Christian
National Research Institute for Agriculture, Food and Environment (INRAE)
Chonova, Teofana
Institut National de la Recherche Agronomique (INRA)
Ciampor, Fedor
Slovak Academy of Sciences
Costa, Filipe O.
Universidade do Minho
Ferrari , Benoit J. D.
Eawag, Swiss Federal Institute of Aquatic Science and Technology
Gastineau, Romain
Maritime University of Szczecin
Goulon, Chloé
National Research Institute for Agriculture, Food and Environment (INRAE)
Gugger, Muriel
Institut Pasteur
Holzmann, Maria
University of Geneva
Jahn, Regine
Free University of Berlin
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Kusber, Wolf-Henning
Free University of Berlin
Laplace-Treyture, Christophe
National Research Institute for Agriculture, Food and Environment (INRAE)
Leese, Florian
University of Duisburg-Essen
Leliaert, Frederik
Botanic Garden Meise
Show more authors

UKÄ Subject classification


Publication Identifiers


URI (permanent link to this page)