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Research article - Peer-reviewed, 2021

Root Rot Resistance Locus PaLAR3 Is Delivered by Somatic Embryogenesis (SE) Pipeline in Norway Spruce (Picea abies (L.) Karst.)

Edesi, Jaanika; Tikkinen, Mikko; Elfstrand, Malin; Olson, Ake; Varis, Saila; Egertsdotter, Ulrika; Aronen, Tuija;

Abstract

Research Highlights: The Norway spruce somatic embryogenesis (SE) pipeline is suitable for multiplication of material with root rot resistance traits. Background and Objectives: Heterobasidion root rot is the economically most severe forest pathogen in Europe, reducing the benefit of planting elite forest material. In this study, the SE-propagation ability of elite Norway spruce material carrying root rot resistance traits was studied. Materials and Methods: We analyzed the presence of the root rot resistance locus PaLAR3B among 80 Finnish progeny-tested Norway spruce plus-trees used for SE-plant production as well as in 241 SE lines (genotypes) derived from them. Seven full-sib families with lines having either AA, AB, or BB genotype for PaLAR3 locus were further studied for their SE-plant propagation ability. Results: The results indicate that 47.5% of the studied elite trees carry the PaLAR3B allele (45% are heterozygous and 2.5% homozygous). The resistance allele was present among the SE lines as expected based on Mendelian segregation and did not interfere with somatic embryo production capacity. All embryos from PaLAR3 genotypes germinated well and emblings were viable in the end of first growing season. However, in three families, PaLAR3B homo- or heterozygotes had 23.2% to 32.1% lower viability compared to their respective hetero- or PaLAR3A homozygotes. Conclusions: There is no trade-off between root rot resistance locus PaLAR3B and somatic embryo production ability, but the allele may interfere with Norway spruce embling establishment.

Keywords

genomic selection; somatic embryogenesis; root rot resistance; vegetative propagation

Published in

Forests

2021, volume: 12, number: 2, article number: 193
Publisher: MDPI

Authors' information

Edesi, Jaanika
Natural Resources Institute Finland (Luke)
Tikkinen, Mikko
Natural Resources Institute Finland (Luke)
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Varis, Saila
Natural Resources Institute Finland (Luke)
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Aronen, Tuija
Natural Resources Institute Finland (Luke)

Associated SLU-program

SLU Network Plant Protection

UKÄ Subject classification

Forest Science

Publication Identifiers

DOI: https://doi.org/10.3390/f12020193

URI (permanent link to this page)

https://res.slu.se/id/publ/111241