Skip to main content
SLU publication database (SLUpub)

Research article2020Peer reviewedOpen access

An ectomycorrhizal fungus alters sensitivity to jasmonate, salicylate, gibberellin, and ethylene in host roots

Basso, Veronica; Kohler, Annegret; Miyauchi, Shingo; Singan, Vasanth; Guinet, Frederic; Simura, Jan; Novak, Ondrej; Barry, Kerrie W.; Amirebrahimi, Mojgan; Block, Jonathan; Daguerre, Yohann; Na, Hyunsoo; Grigoriev, Igor, V; Martin, Francis; Veneault-Fourrey, Claire


The phytohormones jasmonate, gibberellin, salicylate, and ethylene regulate an interconnected reprogramming network integrating root development with plant responses against microbes. The establishment of mutualistic ectomycorrhizal symbiosis requires the suppression of plant defense responses against fungi as well as the modification of root architecture and cortical cell wall properties. Here, we investigated the contribution of phytohormones and their crosstalk to the ontogenesis of ectomycorrhizae (ECM) between grey poplar (Populus tremula x alba) roots and the fungus Laccaria bicolor. To obtain the hormonal blueprint of developing ECM, we quantified the concentrations of jasmonates, gibberellins, and salicylate via liquid chromatography-tandem mass spectrometry. Subsequently, we assessed root architecture, mycorrhizal morphology, and gene expression levels (RNA sequencing) in phytohormone-treated poplar lateral roots in the presence or absence of L. bicolor. Salicylic acid accumulated in mid-stage ECM. Exogenous phytohormone treatment affected the fungal colonization rate and/or frequency of Hartig net formation. Colonized lateral roots displayed diminished responsiveness to jasmonate but regulated some genes, implicated in defense and cell wall remodelling, that were specifically differentially expressed after jasmonate treatment. Responses to salicylate, gibberellin, and ethylene were enhanced in ECM. The dynamics of phytohormone accumulation and response suggest that jasmonate, gibberellin, salicylate, and ethylene signalling play multifaceted roles in poplar L. bicolor ectomycorrhizal development.


cell wall remodelling; crosstalk; defense; ectomycorrhizae; jasmonate; Laccaria bicolor; MiSSP; phytohormones; Populus tremula x alba; symbiosis

Published in

Plant, Cell and Environment
2020, Volume: 43, number: 4, pages: 1047-1068
Publisher: WILEY

      UKÄ Subject classification

      Forest Science

      Publication identifier


      Permanent link to this page (URI)