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Research article - Peer-reviewed, 2020

Evaluation of antimicrobial, antioxidant and cytotoxic properties of bioactive compounds produced from endophytic fungi of Himalayan yew (Taxus wallichiana) in Nepal

Gauchan, Dhurva Prasad; Kandel, Pratistha; Tuladhar, Astha; Acharya, Ashesh; Kadel, Upendra; Baral, Aayush; Shahi, Arjan Bir; Garcia Gil, Rosario

Abstract

Background: Endophytic fungi are largely underexplored in the discovery of natural bioactive products though being rich sources of novel compounds with promising pharmaceutical potential. In this study, Taxus wallichiana, which has huge medicinal value, was investigated for its endophytic diversity and capability to produce bioactive secondary metabolites by analyzing antioxidant, antimicrobial and cytotoxic properties.
Methods: The endophytes were identified by ITS-PCR using genomic DNA samples. The secondary metabolites were extracted by solvent extraction method using ethyl acetate. The antioxidant activity was analyzed by Thin Layer Chromatography, Total Phenol Content (TPC), Total Flavonoid Content (TFC) and DPPH assay, and the antimicrobial activity was analyzed by agar-well diffusion method. Brine shrimp lethality assay was used to analyze the cytotoxicity of the fungal extracts.
Results: Out of 16 different Taxus trees sampled from different locations of Dhorpatan, 13 distinctive endophytic fungi were isolated and grouped into 9 different genera: Bjerkandera, Trichoderma, Preussia, Botrytis, Arthrinium, Alternaria, Cladosporium, Sporormiella and Daldinia. The ethyl acetate extracts isolated from three endophytic fungi: Alternaria alternataCladosporium cladosporioides and Alternaria brassicae showed significant TPC values of 204±6.144, 312.3±2.147 and 152.7±4.958µg GAE/mg of dry extract, respectively, and TFC values of 177.9±2.911, 644.1±4.202 and 96.38±3.851µg RE/mg of dry extract, respectively. Furthermore, these three extracts showed a dose dependent radical scavenging activity with IC50 concentration of 22.85, 22.15 and 23.001 µg/ml, respectively. The extracts of C. cladosporioides and A. brassicae also showed promising antimicrobial activity against Escherichia coliStaphylococcus aureus and Bacillus subtilis with a minimum inhibitory concentration of 250μg/ml for all bacteria. Both the samples showed cytotoxic property against shrimp nauplii with LC50 of 104.2 and 125.9µg/ml, respectively.
Conclusions: The crude fungal extracts obtained from endophytes: A. alternataC. cladosporioides and A. brassicae upon purification and further identification of the bioactive compounds can be a fascinating source for novel pharmaceutical agents.

Keywords

Taxus wallichiana; Endophytic; DPPH; GAE; RE; cytotoxic

Published in

F1000Research
2020, volume: 9, article number: 379

Authors' information

Gauchan, Dhurva Prasad
Kathmandu University
Kandel, Pratistha
Kathmandu University
Tuladhar, Astha
Kathmandu University
Acharya, Ashesh
Kathmandu University
Kadel, Upendra
Kathmandu University
Baral, Aayush
Kathmandu University
Shahi, Arjan Bir
Kathmandu University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Microbiology

Publication Identifiers

DOI: https://doi.org/10.12688/f1000research.23250.2

URI (permanent link to this page)

https://res.slu.se/id/publ/111423