Stymne, Sten
- Department of Crop Science, Swedish University of Agricultural Sciences
Abstract
Despite that a lot of efforts have been devoted to triacylglycerol biosynthesis in plants, we still do not know the number and types of enzymes responsible for triacylglycerol synthesis in oil seeds, not even in the model plant Arabidopsis. The effect of mutations in DGAT2, PDAT1 and PDAT2 genes on seed oil and fatty acid composition in Arabidopsis will be presented as well as in-vitro activities of DGAT and PDAT in membrane preparations from different oil seeds. The results indicate that PDAT may contribute very differently to oil biosynthesis in different species. A soluble and active form of yeast PDAT was obtained by removing the membrane anchor region of the gene which allowed for the characterization of PDAT catalytic activity without interference of endogenous lipids or enzymes. It is shown that the enzyme required glycosylation for activity and could catalyze a variety of different transacylation reactions between various lipids as well as it possessed phospholipase C activity. The PDAT1 gene in Arabidopsis is a member of a family of six genes of which we have shown that one encodes a phospholipid:sterol acyltransferase (PSAT). Results regarding properties of the PSAT enzyme and its physiological functions will be presented. A third Arabidopsis gene of the PDAT family encoded a phospholipase A1 when expressed in yeast but its function in plants is yet not known
Conference
The 17th International Symposium on Plant Lipids
SLU Authors
Carlsson, Anders
- Department of Crop Science, Swedish University of Agricultural Sciences
- Department of Crop Science, Swedish University of Agricultural Sciences
Ståhl, Ulf
- Department of Crop Science, Swedish University of Agricultural Sciences
- Department of Crop Science, Swedish University of Agricultural Sciences
UKÄ Subject classification
Agricultural Science
Food Science
Permanent link to this page (URI)
https://res.slu.se/id/publ/11145