Microbial substrate stoichiometry governs nutrient effects on nitrogen cycling in grassland soils

Schleuss, P. M.; Widdig, M.; Biederman, L. A.; Borer, E. T.; Crawley, M. J.; Kirkman, K. P.; Seabloom, E. W.; Wragg, P. D.; Spohn, M.

doi:10.1016/j.soilbio.2021.108168

Abstract

Human activities have increased nitrogen (N) and phosphorus (P) inputs in terrestrial ecosystems and altered carbon (C) availability, shifting the stoichiometry of microbial substrates in soils, such as the C:N:P ratios of the dissolved organic matter pool. These stoichiometric deviations between microbial biomass and its substrate may control microbial processes of N cycling. We studied the effects of this stoichiometric mismatch using a full factorial N and P addition experiment replicated in six grassland ecosystems in South Africa, the USA, and the UK. We found that N and P addition changed the dissolved organic matter C:N ratio, but not the C:N ratio of the soil microbial biomass. Compared to P addition, N addition decreased microbial N acquisition via non-symbiotic N-2 fixation by -55% and increased microbial N release via net N mineralization by +134%. A possible explanation is that the dissolved elements, e.g., dissolved organic C (DOC) and dissolved total N (DN), serve as the main microbial substrate and its C:N ratio defines whether N is scarce or abundant with respect to microbial demands. If N is available in excess relative to microbial demands, net N mineralization increases. In contrast, when N is scarce, immobilization outweighs release decreasing net N mineralization. However, the activity of leucine aminopeptidases, which decompose peptides, was not affected by nutrient additions. Further, C rather than P availability may control the rates of non-symbiotic N-2 fixation in the six studied grassland sites. In conclusion, globally increasing nutrient inputs change processes of microbial N acquisition and release in grassland ecosystems and these changes are largely driven by shifts in substrate stoichiometry.

Keywords

Nitrogen release and acquisition; Net N mineralization; Non-symbiotic N-2 fixation; Leucine aminopeptidase activity; Nutrient fertilization; Ecological Stoichiometry; Nutrient Network (NutNet)

Published in

Soil Biology and Biochemistry
2021, volume: 155, article number: 108168
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

SLU Authors

Spohn, Marie
- Department of Soil and Environment, Swedish University of Agricultural Sciences
- University of Bayreuth

Global goals (SDG)

SDG15 Life on land

UKÄ Subject classification

Soil Science

Publication identifier

DOI: https://doi.org/10.1016/j.soilbio.2021.108168

Permanent link to this page (URI)

https://res.slu.se/id/publ/111481