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Research article - Peer-reviewed, 2021

Single-Shot Vaccines against Bovine Respiratory Syncytial Virus (BRSV): Comparative Evaluation of Long-Term Protection after Immunization in the Presence of BRSV-Specific Maternal Antibodies

Valarcher, Jean Francois; Hagglund, Sara; Naslund, Katarina; Jouneau, Luc; Malmstrom, Ester; Boulesteix, Olivier; Pinard, Anne; Leguere, Dany; Deslis, Alain; Gauthier, David; Dubuquoy, Catherine; Pietralunga, Vincent; Remot, Aude; Falk, Alexander; Shevchenko, Ganna; Bergstrom Lind, Sara; Von Bromssen, Claudia; Vargmar, Karin; Zhang, Baoshan; Kwong, Peter D.;
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Abstract

The induction of long-lasting clinical and virological protection is needed for a successful vaccination program against the bovine respiratory syncytial virus (BRSV). In this study, calves with BRSV-specific maternally derived antibodies were vaccinated once, either with (i) a BRSV pre-fusion protein (PreF) and Montanide(TM) ISA61 VG (ISA61, n = 6), (ii) BRSV lacking the SH gene (Delta SHrBRSV, n = 6), (iii) a commercial vaccine (CV, n = 6), or were injected with ISA61 alone (n = 6). All calves were challenged with BRSV 92 days later and were euthanized 13 days post-infection. Based on clinical, pathological, and proteomic data, all vaccines appeared safe. Compared to the controls, PreF induced the most significant clinical and virological protection post-challenge, followed by Delta SHrBRSV and CV, whereas the protection of PreF-vaccinated calves was correlated with BRSV-specific serum immunoglobulin (Ig)G antibody responses 84 days post-vaccination, and the IgG antibody titers of Delta SHrBRSV- and CV-vaccinated calves did not differ from the controls on this day. Nevertheless, strong anamnestic BRSV- and PreF-specific IgG responses occurred in calves vaccinated with either of the vaccines, following a BRSV challenge. In conclusion, PreF and Delta SHrBRSV are two efficient one-shot candidate vaccines. By inducing a protection for at least three months, they could potentially improve the control of BRSV in calves.

Keywords

respiratory syncytial virus; bovine; neonatal; subunit vaccine; pre-fusion; live-attenuated vaccine; deleted SHrBRSV; one-shot; duration of protection; correlate of protection

Published in

Vaccines
2021, volume: 9, number: 3, article number: 236
Publisher: MDPI

Authors' information

Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Jouneau, Luc
Universite Paris Saclay
Malmström, Ester
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Boulesteix, Olivier
INRAE
Pinard, Anne
INRAE
Leguere, Dany
INRAE
Deslis, Alain
INRAE
Gauthier, David
INRAE
Dubuquoy, Catherine
Universite Paris Saclay
Pietralunga, Vincent
Universite Paris Saclay
Remot, Aude
Universite de Tours
Falk, Alexander
Uppsala University
Shevchenko, Ganna
Uppsala University
Bergstrom Lind, Sara
Uppsala University
Swedish University of Agricultural Sciences, Department of Energy and Technology
Zhang, Baoshan
NIH National Institute of Allergy and Infectious Diseases (NIAID)
Kwong, Peter D.
NIH National Institute of Allergy and Infectious Diseases (NIAID)
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UKÄ Subject classification

Clinical Science

Publication Identifiers

DOI: https://doi.org/10.3390/vaccines9030236

URI (permanent link to this page)

https://res.slu.se/id/publ/111627