Abstract

Accurate prediction of the temperature response of the velocity v of a biochemical reaction has wide applications in cell biology, reaction design, and biomass yield enhancement. Here, we introduce a simple but comprehensive mechanistic approach that uses thermodynamics and biochemical kinetics to describe and link the reaction rate and Michaelis-Menten constants (k(T) and K-T) with the biomass yield and mortality rate (Y-T and (T)) as explicit functions of T. The temperature control is exerted by catabolic enthalpy at low temperatures and catabolic entropy at high temperatures, whereas changes in cell and enzyme-substrate heat capacity shift the anabolic electron use efficiency e(A) and the maximum reaction velocity v(max). We show that cells have optimal growth when the catabolic (differential) free energy of activation decreases the cell free energy harvest required to duplicate their internal structures as long as electrons for anabolism are available. With the described approach, we accurately predicted observed glucose fermentation and ammonium nitrification dynamics across a wide temperature range with a minimal number of thermodynamics parameters, and we highlight how kinetic parameters are linked to each other using first principles.

Published in

International Journal of Chemical Kinetics
2018, Volume: 50, number: 5, pages: 343-356
Publisher: WILEY

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1002/kin.21163

Permanent link to this page (URI)

https://res.slu.se/id/publ/111716