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Research article - Peer-reviewed, 2021

Pesticide mixture toxicity to algae in agricultural streams – Field observations and laboratory studies with in situ samples and reconstituted water

Stenstrom, Jenny Rydh; Kreuger, Jenny; Goedkoop, Willem

Abstract

Long-term pesticide water concentrations were investigated in four agricultural streams and their mixture toxicity on algae was assessed, based on realistic (i.e. observed) concentrations in laboratory tests using (i) natural weekly water samples and (ii) reconstituted pesticide-spiked water samples representing mixtures with predicted high mixture. This approach both covered the full complexity of natural water samples and the controlled approach of reconstituted water samples. Long-term monitoring data (time-integrated, weekly samples) revealed more than 11 pesticides (range 11.0 ? 0.25?24.0 ? 0.44) in 75% or more of the almost 1600 samples collected between 2002 and 2018. ETUalgae exceeded 0.1 for 29 observations (or 1.8%). Despite the multitude of pesticides in a sample, ETUalgae was frequently set by one or a few dominating pesticides that contribute to more than 90% of the mixture?s toxicity. Algal growth inhibition tests with in situ stream water showed a high frequency of inhibition, despite the low ETU for most of these samples (range 0.000014?0.3858). These ?false positive? results were attributed to confounding effects of turbidity, the complexation of nutrients, and toxic effects of metals and/or other unknown contaminants. Algal inhibition tests with spiked reconstituted water showed significant inhibitory effects in the range of 1?10x the ETUalgae observed in worst-case field samples. Although these tests disregard the chemical complexity of natural water, they show that inhibitory effects of pesticides on algae may occur at the ETUalgae observed in monitoring. Furthermore, considering that the ETUalgae of stream water are based on weekly average concentrations and likely underestimate short-term peak concentrations of pesticides, these results strongly suggest that inhibitory effects on algae may occur in the agricultural streams of southern Sweden. We conjecture, however, that the rapid recovery of algae contributes to ameliorate these short-term effects and that pesticide contamination should be seen as one of many stressors in the streams that drain agricultural landscapes.

Keywords

Pesticide mixture toxicity; Algae; Growth inhibition; Long-term monitoring; Streams; Laboratory toxicity tests

Published in

Ecotoxicology and Environmental Safety
2021, volume: 215, article number: 112153
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

Authors' information

Rydh Stenström, Jenny
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Kreuger, Jenny
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment

Sustainable Development Goals

SDG6 Clean water

UKÄ Subject classification

Environmental Sciences

Publication Identifiers

DOI: https://doi.org/10.1016/j.ecoenv.2021.112153

URI (permanent link to this page)

https://res.slu.se/id/publ/111748